Defatted Wheat Germ Protein-Derived Peptides Showed Multiple Biological Activities from the Stomach to Small Intestine: In Silico and In Vitro Approaches,Journal of Agricultural and Food Chemistry

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Defatted Wheat Germ Protein-Derived Peptides Showed Multiple Biological Activities from the Stomach to Small Intestine: In Silico and In Vitro Approaches
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-04 , DOI: 10.1021/acs.jafc.4c06539
Bolappa Gamage Kaushalya Madhavi ₁ , Anushi Madushani Wijethunga ₁ , Ogadimma D Okagu ₂ , Xiaohong Sun ₁

Affiliation

This study aimed to test the hypothesis that bioactive peptides can exert multiple bioactivities at different sites in the gastrointestinal tract. Our previous research identified 33 gastric-resistant peptides derived from wheat germ with potential antiadhesive activity against Helicobacter pylori in the stomach. In this work, in silico digestion of these peptides with trypsin, thermolysin, and chymotrypsin produced 67 peptide fragments. Molecular docking was conducted to predict their ACE and DPP-IV inhibitory activities in the small intestine. Three peptides (VPIPNPSGDR, VPY, and AR) were selected and synthesized for in vitro validation. Their generation in the gastrointestinal tract was verified via in vitro digestion, followed by mass spectrometry analysis. The IC₅₀ values for ACE inhibition were 199.5 μM (VPIPNPSGDR), 316.3 μM (VPY), and 446.7 μM (AR). For DPP-IV inhibition, their IC₅₀ values were 0.5, 1.6, and 4.0 mM, respectively. This research pioneers new directions in the emerging field of multifunctional peptides, providing scientific evidence to support the utilization of wheat germ as value-added food ingredients.

中文翻译：

脱脂小麦胚芽蛋白衍生肽显示出从胃到小肠的多种生物活性：计算机模拟和体外方法

本研究旨在检验生物活性肽可以在胃肠道不同部位发挥多种生物活性的假设。我们之前的研究鉴定出 33 种源自小麦胚芽的胃抗性肽，对胃中的幽门螺杆菌具有潜在的抗粘附活性。在这项工作中，用胰蛋白酶、嗜热菌蛋白酶和胰凝乳蛋白酶对这些肽进行计算机模拟消化，产生了 67 个肽片段。进行分子对接以预测它们在小肠中的 ACE 和 DPP-IV 抑制活性。选择并合成了三种肽（VPIPNPSGDR、VPY 和 AR）进行体外验证。通过体外消化和质谱分析验证了它们在胃肠道中的产生。 ACE抑制的IC ₅₀值为199.5 μM (VPIPNPSGDR)、316.3 μM (VPY)和446.7 μM (AR)。对于DPP-IV抑制，它们的IC ₅₀值分别为0.5、1.6和4.0 mM。该研究开创了多功能肽新兴领域的新方向，为支持小麦胚芽作为增值食品成分的利用提供了科学证据。

更新日期：2024-09-04

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