The study focuses on the critically endangered Dabry’s sturgeon (Acipenser dabryanus), a species on the brink of extinction in the wild. This research investigates the role of the Forkhead box protein L2 (foxl2) in the gonadal development and differentiation of this species. The foxl2 gene, known for its role in various physiological processes, including sexual maturation, is hypothesized to play a significant role in the sex differentiation of Dabry’s sturgeon. This study cloned the full-length cds sequence of the foxl2 gene and analyzed its expression across various tissues, focusing on its response to estradiol treatment. Our findings indicate that foxl2 is predominantly expressed in ovaries and shows a dose-dependent response to estradiol, suggesting its potential role in ovarian differentiation. This research underscores the importance of foxl2 in understanding reproductive biology and offers a foundation for future conservation strategies.

中文翻译：

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达氏鲟foxl2基因的克隆、鉴定、功能分析及17β-雌二醇(E2)处理后的表达


该研究的重点是极度濒危的达布里鲟（Acipenser dabryanus），这是一种在野外濒临灭绝的物种。本研究调查叉头盒蛋白 L2 (foxl2) 在该物种性腺发育和分化中的作用。 Foxl2基因因其在包括性成熟在内的各种生理过程中的作用而闻名，据推测在达布里鲟的性别分化中发挥着重要作用。这项研究克隆了foxl2基因的全长cds序列，并分析了其在不同组织中的表达，重点关注其对雌二醇治疗的反应。我们的研究结果表明，foxl2 主要在卵巢中表达，并对雌二醇表现出剂量依赖性反应，表明其在卵巢分化中的潜在作用。这项研究强调了foxl2在理解生殖生物学方面的重要性，并为未来的保护策略奠定了基础。