Double-Wing Motif Protein is a Novel Biofilm Regulatory Factor of the Plant Disease Biocontrol Agent, Bacillus subtilis,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Double-Wing Motif Protein is a Novel Biofilm Regulatory Factor of the Plant Disease Biocontrol Agent, Bacillus subtilis
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-03 , DOI: 10.1021/acs.jafc.4c02192
Qianqian Dong ₁ , Yinghang Chang _{1,

2} , Paul H Goodwin ₃ , Qingxiang Liu _{1,

2} , Wen Xu ₁ , Mingcong Xia ₁ , Jie Zhang ₁ , Runhong Sun ₁ , Shuxia Xu ₂ , Chao Wu ₁ , Kun Wu ₂ , Lirong Yang ₁

Affiliation

Transposon mutagenesis screening of Bacillus subtilis YB-1471, a novel rhizosphere biocontrol agent of Fusarium crown rot (FCR) of wheat, resulted in the identification of orf04391, linked to reduced biofilm formation. The gene encodes a protein possessing a putative tertiary structure of a “double-wing” DNA-binding domain. Expression of orf04391 increased during biofilm development in stationary cultures and during rapid growth in shaking cultures. An orf04391 deletion strain showed reduced biofilm production related to lower levels of the extracellular matrix, and the mutant also had reduced sporulation, adhesion, root colonization, and FCR biocontrol efficiency. Transcriptome analysis of YB-1471 and Δorf04391 in stationary culture showed that the loss of orf04391 resulted in altered expression of numerous genes, including sinI, an initiator of biofilm formation. DNA binding was shown with his-tagged Orf04391 binding to the sinIR operon in vivo and in vitro. Orf04391 appears to be a transcriptional regulator of biofilm formation in B. subtilis through the Spo0A-SinI/SinR pathway.

中文翻译：

双翼基序蛋白是植物病害生物防治剂枯草芽孢杆菌的新型生物膜调节因子

对枯草芽孢杆菌YB-1471（一种小麦镰孢菌冠腐病 (FCR) 的新型根际生物防治剂）进行转座子诱变筛选，结果鉴定出orf04391 ，与减少生物膜形成有关。该基因编码的蛋白质具有“双翼”DNA 结合域的假定三级结构。 orf04391的表达在静止培养物中的生物膜发育过程中以及在摇动培养物中的快速生长过程中增加。 orf04391缺失菌株表现出与细胞外基质水平较低相关的生物膜产生减少，并且该突变体还具有减少的孢子形成、粘附、根定植和FCR生物防治效率。静止培养中 YB-1471 和 Δorf04391 的转录组分析表明， orf04391的缺失导致许多基因的表达改变，包括sinI （生物膜形成的启动子）。在体内和体外，DNA 结合显示为带有 his 标记的 Orf04391 与sinIR操纵子的结合。 Orf04391 似乎是枯草芽孢杆菌中通过 Spo0A-SinI/SinR 途径形成生物膜的转录调节因子。

更新日期：2024-09-03

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南