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Engineering Entomopathogenic Fungi Using Thermal-Responsive Polymer to Boost Their Resilience against Abiotic Stresses
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-03 , DOI: 10.1021/acs.jafc.4c04400 Guang Yang 1, 2, 3 , Feihu Bi 1 , Deshui Yu 2 , Yulong Wang 2 , Hui Ren 1 , Hanchen Wei 1 , Zhangxun Wang 4 , Bo Huang 2
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-03 , DOI: 10.1021/acs.jafc.4c04400 Guang Yang 1, 2, 3 , Feihu Bi 1 , Deshui Yu 2 , Yulong Wang 2 , Hui Ren 1 , Hanchen Wei 1 , Zhangxun Wang 4 , Bo Huang 2
Affiliation
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Entomopathogenic fungi offer an ecologically sustainable and highly effective alternative to chemical pesticides for managing plant pests. However, the efficacy of mycoinsecticides in pest control suffers from environmental abiotic stresses, such as solar UV radiation and temperature fluctuations, which seriously hinder their practical application in the field. Herein, we discovered that the synthetic amphiphilic thermal-responsive polymers are able to significantly enhance the resistance of Metarhizium robertsii conidia against thermal and UV irradiation stresses. The thermosensitive polymers with extremely low cytotoxicity and good biocompatibility can be engineered onto the M. robertsii conidia surface by anchoring hydrophobic alkyl chains. Further investigations revealed that polymer supplementation remarkably augmented the capacity for penetration and the virulence of M. robertsii under heat and UV stresses. Notably, broad-spectrum entomopathogenic fungi can be protected by the polymers. The molecular mechanism was elucidated through exploring RNA sequencing and in vivo/vitro enzyme activity assays. This work provides a novel avenue for fortifying the resilience of entomopathogenic fungi, potentially advancing their practical application as biopesticides.
中文翻译：
使用热响应聚合物改造昆虫病原真菌以增强其对非生物胁迫的恢复能力
昆虫病原真菌为防治植物害虫提供了一种生态可持续且高效的化学农药替代品。然而，霉菌杀虫剂在害虫防治方面的功效受到环境非生物胁迫的影响，例如太阳紫外线辐射和温度波动，这严重阻碍了其在该领域的实际应用。在此，我们发现合成的两亲性热响应聚合物能够显着增强绿僵菌分生孢子对热和紫外线照射应激的抵抗力。通过锚定疏水性烷基链，可以将具有极低细胞毒性和良好生物相容性的热敏聚合物工程化到罗氏分生孢子表面。进一步的研究表明，聚合物补充剂显着增强了罗伯氏分枝杆菌在热和紫外线胁迫下的渗透能力和毒力。值得注意的是，聚合物可以保护广谱昆虫病原真菌。通过探索RNA测序和体内/体外酶活性测定阐明了分子机制。这项工作为增强昆虫病原真菌的抵抗力提供了一条新途径，有可能促进其作为生物农药的实际应用。
更新日期：2024-09-03
中文翻译：
使用热响应聚合物改造昆虫病原真菌以增强其对非生物胁迫的恢复能力
昆虫病原真菌为防治植物害虫提供了一种生态可持续且高效的化学农药替代品。然而，霉菌杀虫剂在害虫防治方面的功效受到环境非生物胁迫的影响，例如太阳紫外线辐射和温度波动，这严重阻碍了其在该领域的实际应用。在此，我们发现合成的两亲性热响应聚合物能够显着增强绿僵菌分生孢子对热和紫外线照射应激的抵抗力。通过锚定疏水性烷基链，可以将具有极低细胞毒性和良好生物相容性的热敏聚合物工程化到罗氏分生孢子表面。进一步的研究表明，聚合物补充剂显着增强了罗伯氏分枝杆菌在热和紫外线胁迫下的渗透能力和毒力。值得注意的是，聚合物可以保护广谱昆虫病原真菌。通过探索RNA测序和体内/体外酶活性测定阐明了分子机制。这项工作为增强昆虫病原真菌的抵抗力提供了一条新途径，有可能促进其作为生物农药的实际应用。
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