Molecular Detection of kdr and superkdr Mutation Sites and Analysis of the Binding Modes of Pyrethroid Insecticides with Voltage-Gated Sodium Channels in the Plant Bug Lygus pratensis (Hemiptera: Miridae),Journal of Agricultural and Food Chemistry

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Molecular Detection of kdr and superkdr Mutation Sites and Analysis of the Binding Modes of Pyrethroid Insecticides with Voltage-Gated Sodium Channels in the Plant Bug Lygus pratensis (Hemiptera: Miridae)
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-03 , DOI: 10.1021/acs.jafc.4c03416
Liqi Zhang _{1,

2} , Ruoyao Ni ₃ , Jing Chen _{1,

2} , Jiale Yang _{1,

2} , Yawen Dong ₄ , Zhiguang Yuchi ₅ , Yao Tan _{1,

2,

3}

Affiliation

This study identified genetic mutations linked to resistance to pyrethroid insecticides in the plant pest Lygus pratensis. The voltage-gated sodium channel (VGSC) gene was cloned, revealing two mutations (Met918Thr and Leu1014Phe) in laboratory strains and field populations from Inner Mongolia, resulting in variable pyrethroid resistance. A 3D model of LpVGSC was created using homology modeling, and pyrethroid binding patterns were analyzed via molecular docking. Molecular dynamics simulations confirmed structural stability changes and binding stability of pyrethroids to VGSC sites. Mutation frequencies of homozygous and heterozygous genotypes did not exceed 40 and 20%, respectively. Toxicity tests showed high resistance to λ-cyhalothrin (LC50:401.31 ng/cm²). The kdr (L1014F) and superkdr (M918T) mutations weakened interaction forces, reducing pyrethroid binding. M918T and L1014F mutations are predicted to reduce Type I pyrethroid affinity, suggesting Type II pyrethroids may be more effective against resistant strains. These findings aid in resistance management and insecticide design.

中文翻译：

植物臭虫 Lygus pratensis（半翅目：Miridae）中 kdr 和 superkdr 突变位点的分子检测及拟除虫菊酯类杀虫剂与电压门控钠通道的结合模式分析

这项研究确定了植物害虫 Lygus pratensis 中与对拟除虫菊酯类杀虫剂的抗药性相关的基因突变。克隆电压门控钠通道（VGSC）基因，揭示了内蒙古实验室菌株和现场种群中的两个突变（Met918Thr 和 Leu1014Phe），导致不同的拟除虫菊酯耐药性。使用同源建模创建 LpVGSC 的 3D 模型，并通过分子对接分析拟除虫菊酯结合模式。分子动力学模拟证实了拟除虫菊酯的结构稳定性变化和与 VGSC 位点的结合稳定性。纯合子和杂合子基因型的突变频率分别不超过 40% 和 20%。毒性试验显示对 λ-氯氟氰菊酯（LC50：401.31 ng/cm²）具有高度耐药性。kdr （L1014F）和 superkdr （M918T）突变减弱了相互作用力，减少了拟除虫菊酯的结合。预计 M918T 和 L1014F 突变会降低 I 型拟除虫菊酯亲和力，表明 II 型拟除虫菊酯可能对耐药菌株更有效。这些发现有助于耐药性和杀虫剂设计。

更新日期：2024-09-03

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