The Fat Body-Specific GST Gene SlGSTe11 Enhances the Tolerance of Spodoptera litura to Cyantraniliprole and Nicotine,Journal of Agricultural and Food Chemistry

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The Fat Body-Specific GST Gene SlGSTe11 Enhances the Tolerance of Spodoptera litura to Cyantraniliprole and Nicotine
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-03 , DOI: 10.1021/acs.jafc.4c05747
Long Jin ₁ , Kunpeng Yan ₁ , Haoran Kong ₁ , Jianyi Li ₁ , Chengcheng Fan ₁ , Yiou Pan ₁ , Qingli Shang ₁

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Spodoptera litura is a significant agricultural pest, and its glutathione S-transferase (GST) plays a crucial role in insecticide resistance. This study aimed to investigate the relationship between the SlGSTe11 gene of S. litura and resistance to cyantraniliprole and nicotine. Transcriptome analysis revealed that SlGSTe11 is highly expressed mainly in fat bodies, with a significant increase in SlGSTe11 gene expression under induction by cyantraniliprole and nicotine. The ectopic expression of the SlGSTe11 gene in transgenic fruit flies resulted in a 5.22-fold increase in the tolerance to cyantraniliprole. Moreover, compared to the UAS-SlGSTe11 line, the Act5C-UAS>SlGSTe11 line laid more eggs and had a lower mortality after nicotine exposure. RNAi-mediated inhibition of SlGSTe11 gene expression led to a significant increase in the mortality of S. litura under cyantraniliprole exposure. In vitro metabolism experiments demonstrated that the recombinant SlGSTe11 protein efficiently metabolizes cyantraniliprole. Molecular docking results indicated that SlGSTe11 has a strong affinity for both cyantraniliprole and nicotine. These findings suggest that SlGSTe11 is involved in the development of resistance to cyantraniliprole and nicotine in S. litura.

中文翻译：

脂肪体特异性 GST 基因 SlGSTe11 增强了斜纹夜蛾对氰虫酰胺和尼古丁的耐受性

斜纹夜蛾是一种重要的农业害虫，其谷胱甘肽 S-转移酶（GST）在杀虫剂抗性中起着至关重要的作用。本研究旨在探讨 S. litura 的 SlGSTe11 基因与对氰虫酰胺和尼古丁的耐药性之间的关系。转录组分析显示，SlGSTe11 主要在脂肪体中高表达，在氰虫酰胺和尼古丁诱导下 SlGSTe11 基因表达显著增加。转基因果蝇中 SlGSTe11 基因的异位表达导致对氰虫酰胺的耐受性增加 5.22 倍。此外，与 UAS-SlGSTe11 系相比，Act5C-UAS>SlGSTe11 系在尼古丁暴露后产卵更多，死亡率更低。RNAi 介导的 SlGSTe11 基因表达抑制导致氰虫酰胺暴露下 S. litura 的死亡率显着增加。体外代谢实验表明，重组 SlGSTe11 蛋白可有效代谢氰虫酰胺。分子对接结果表明，SlGSTe11 对氰虫酰胺和尼古丁均具有很强的亲和力。这些发现表明 SlGSTe11 参与 S. litura 对氰虫酰胺和尼古丁耐药性的发展。

更新日期：2024-09-03

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