Overexpression of Two Odorant Binding Proteins Confers Chlorpyrifos Resistance in the Green Peach Aphid Myzus persicae,Journal of Agricultural and Food Chemistry

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Overexpression of Two Odorant Binding Proteins Confers Chlorpyrifos Resistance in the Green Peach Aphid Myzus persicae
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-02 , DOI: 10.1021/acs.jafc.4c05026
Xing Xiao ₁ , Xin-Hui Yin ₁ , Shi-Yuan Hu ₁ , Hao-Nan Miao ₁ , Zhuo Wang _{1,

2} , Hu Li ₁ , Yong-Jun Zhang ₃ , Pei Liang ₁ , Shao-Hua Gu _{1,

2}

Affiliation

The green peach aphid, Myzus persicae, is a worldwide agricultural pest. Chlorpyrifos has been widely used to control M. persicae for decades, thus leading to a high resistance to chlorpyrifos. Recent studies have found that insect odorant binding proteins (OBPs) play essential roles in insecticide resistance. However, the potential resistance mechanism underlying the cross-link between aphid OBPs and chlorpyrifos remains unclear. In this study, two OBPs (MperOBP3 and MperOBP7) were found overexpressed in M. persicae chlorpyrifos-resistant strains (CRR) compared to chlorpyrifos-sensitive strains (CSS); furthermore, chlorpyrifos can significantly induce the expression of both OBPs. An in vitro binding assay indicated that both OBPs strongly bind with chlorpyrifos; an in vivo RNAi and toxicity bioassay confirmed silencing either of the two OBPs can increase the susceptibility of aphids to chlorpyrifos, suggesting that overexpression of MperOBP3 and MperOBP7 contributes to the development of resistance of M. persicae to chlorpyrifos. Our findings provide novel insights into insect OBPs-mediated resistance mechanisms.

中文翻译：

两种气味结合蛋白的过度表达赋予绿桃蚜虫对毒死蜱的抗性

桃蚜（ Myzus persicae ）是一种世界性的农业害虫。几十年来，毒死蜱已被广泛用于控制桃小霉，从而导致对毒死蜱的高抗性。最近的研究发现昆虫气味结合蛋白（OBP）在杀虫剂抗性中发挥着重要作用。然而，蚜虫 OBP 和毒死蜱之间的潜在抗性机制仍不清楚。在这项研究中，与毒死蜱敏感菌株（CSS）相比，两种 OBP（MperOBP3 和 MperOBP7）在桃毒死蜱抗性菌株（CRR）中过度表达。此外，毒死蜱可以显着诱导两种OBPs的表达。体外结合试验表明，两种 OBP 均与毒死蜱强烈结合；体内RNAi和毒性生物测定证实，沉默这两个OBP中的任何一个都可以增加蚜虫对毒死蜱的敏感性，这表明MperOBP3和MperOBP7的过度表达有助于桃蚜对毒死蜱的抗性的发展。我们的研究结果为昆虫 OBP 介导的抗性机制提供了新的见解。

更新日期：2024-09-02

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