Production of functional biocompounds from microalgae has garnered interest from different industrial sectors; however, their overall productivity must be substantially improved for commercialization. Herein, long-term acclimation of Scenedesmus deserticola was conducted using glucose as an organic carbon source to enhance its heterotrophic capabilities and the production potential of loliolide. A year-long acclimation on agar plates led to the selection of S. deserticola HS4, which exhibited at least 2-fold increase in loliolide production potential; S. deserticola HS4 was subjected to further screening of its cultivation conditions and fed-batch cultivation was subsequently performed in liter-scale reactors. While S. deserticola HS4 exhibited shifts in cellular morphology and biochemical composition, the results suggested a substantial increase in its loliolide productivity regardless of trophic modes. Collectively, these results highlight the potential of long-term acclimation as an effective strategy for improving microalgal crops to align with industrial production practices.

中文翻译：

---

对有机碳的长期适应提高了沙漠栅藻中黑麦内酯的产量


利用微藻生产功能性生物化合物引起了不同工业部门的兴趣；然而，为了实现商业化，必须大幅提高其整体生产力。本文以葡萄糖为有机碳源对沙漠栅藻进行长期驯化，以增强其异养能力和黑麦内酯的生产潜力。经过在琼脂平板上长达一年的驯化，我们选择了 S. Deserticola HS4，它的黑麦草内酯生产潜力至少增加了 2 倍；对 S. Deserticola HS4 的培养条件进行了进一步筛选，随后在升级反应器中进行补料分批培养。虽然 S. Deserticola HS4 表现出细胞形态和生化组成的变化，但结果表明，无论营养模式如何，其黑麦内酯生产力均显着增加。总的来说，这些结果凸显了长期驯化作为改善微藻作物以符合工业生产实践的有效策略的潜力。