INTRODUCTION Chronic kidney disease (CKD) poses a significant global health burden, with increasing prevalence and high morbidity and mortality rates, particularly in end-stage kidney disease (ESKD). While traditional risk factors contribute, the exact mechanisms remain elusive, with inflammation playing a pivotal role. Medium cut-off (MCO) membranes offer promise in improving dialysis outcomes by efficiently clearing uremic toxins without substantial albumin loss. We aimed to elucidate the impact of MCO and high-flux (HF) membranes on peripheral blood lymphocyte subpopulations in hemodialysis patients. METHODS Twenty-four ESKD patients underwent 36 sessions each with MCO and HF membranes. Immunophenotyping by flow cytometry was performed to analyze lymphocyte subsets. RESULTS NK cell percentages significantly increased with MCO, returning to baseline with HF. Th1 cells decreased post-HF, while Th2 and TFH cells increased with MCO and persisted. Treg cells remained stable with MCO but decreased with HF. CONCLUSION MCO dialysis induced an anti-inflammatory shift, evidenced by increased Th2 and TFH cells and stable Treg cells. NK cells also responded favorably to MCO. These findings underscore MCO membranes' potential to modulate immune responses and improve patient outcomes in ESKD.

中文翻译：

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使用中截止膜和高通量膜进行血液透析的患者的外周血淋巴细胞亚群：SHE 继续研究。


简介 慢性肾病（CKD）对全球健康造成重大负担，其患病率不断增加，发病率和死亡率也很高，尤其是终末期肾病（ESKD）。虽然传统的危险因素有所贡献，但确切的机制仍然难以捉摸，其中炎症发挥着关键作用。中截止 (MCO) 膜通过有效清除尿毒症毒素而不会造成大量白蛋白损失，有望改善透析结果。我们的目的是阐明 MCO 和高通量 (HF) 膜对血液透析患者外周血淋巴细胞亚群的影响。方法 24 名 ESKD 患者接受了 36 次 MCO 和 HF 膜疗程。通过流式细胞术进行免疫表型分析来分析淋巴细胞亚群。结果 MCO 时 NK 细胞百分比显着增加，HF 时恢复到基线。 HF 后 Th1 细胞减少，而 Th2 和 TFH 细胞随 MCO 增加并持续存在。 Treg 细胞在 MCO 中保持稳定，但在 HF 中减少。结论 MCO 透析诱导抗炎转变，Th2 和 TFH 细胞增加以及 Treg 细胞稳定即可证明。 NK 细胞对 MCO 也有良好的反应。这些发现强调了 MCO 膜调节免疫反应和改善 ESKD 患者预后的潜力。