Marine thermal fluctuation profoundly influences energy metabolism, physiology, and survival of marine life. In the present study, short-term and long-term high-temperature stresses were found to affect gluconeogenesis by inhibiting PEPCK activity in the Pacific oyster (Crassostrea gigas), which is a globally distributed species that encounters significant marine thermal fluctuations in intertidal zones worldwide. CgCREBL2, a key molecule in the regulation of gluconeogenesis, plays a critical role in the transcriptional regulation of PEPCK in gluconeogenesis against high-temperature stress. CgCREBL2 was able to increase the transcription of CgPEPCK by either binding the promoter of CgPEPCK gene or activating CgPGC-1α and CgHNF-4α after short-term (6 h) high-temperature stress, while only by binding CgPEPCK after long-term (60 h) high-temperature stress. These findings will further our understanding of the effect of marine thermal fluctuation on energy metabolism on marine organisms.

中文翻译：

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海洋热波动通过太平洋牡蛎 CgCREBL2 的转录调控诱导糖异生


海洋热波动深刻影响海洋生物的能量代谢、生理和生存。在本研究中，发现短期和长期高温胁迫通过抑制太平洋牡蛎 （Crassostrea gigas） 的 PEPCK 活性来影响糖异生，太平洋牡蛎 （Crassostrea gigas） 是一种全球分布的物种，在全球潮间带遇到显着的海洋热波动。CgCREBL2 是糖异生调控的关键分子，在糖异生对抗高温胁迫的 PEPCK 转录调控中起关键作用。CgCREBL2 在短期 （6 h） 高温胁迫后通过结合 CgPEPCK 基因的启动子或激活 CgPGC-1α 和 CgHNF-4α 来增加 CgPEPCK 的转录，而仅在长期 （60 h） 高温胁迫后通过结合 CgPEPCK 来增加转录。这些发现将进一步了解海洋热波动对海洋生物能量代谢的影响。