Despite increasing concerns regarding the interactions of microplastic and heavy metal pollution, there is limited knowledge on the molecular responses of marine organisms to these stressors. In this study, we used whole-transcriptome sequencing to investigate the molecular responses of the ecologically and economically important bivalve to individual and combined exposures of environmentally relevant concentrations of PVC microplastics and cadmium (Cd). Our results revealed distinct transcriptional changes in , with significant overlap in the differentially expressed genes between the individual and combined exposure groups. Genes involved in cellular senescence, oxidative stress, and galactose metabolism were differentially expressed. Additionally, key signaling pathways related to apoptosis and drug metabolism were significantly modulated. Notably, the interaction of PVC microplastics and Cd resulted in differential expression of genes involved in drug metabolism and longevity regulating compared to single exposures. This suggests that the interaction between these two stressors may have amplified effects on mussel health. Overall, this comprehensive transcriptomic analysis provides valuable insights into the adaptive and detrimental responses of to PVC microplastics and Cd in the environment.

中文翻译：

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转录组分析揭示了聚氯乙烯微塑料和镉对贻贝的相互作用影响：洞察非编码 RNA 反应和环境影响


尽管人们越来越关注微塑料和重金属污染的相互作用，但人们对海洋生物对这些压力源的分子反应的了解有限。在这项研究中，我们使用全转录组测序来研究具有生态和经济重要性的双壳类动物对环境相关浓度的 PVC 微塑料和镉 (Cd) 的单独和组合暴露的分子反应。我们的结果揭示了明显的转录变化，个体和组合暴露组之间的差异表达基因显着重叠。参与细胞衰老、氧化应激和半乳糖代谢的基因存在差异表达。此外，与细胞凋亡和药物代谢相关的关键信号通路也受到显着调节。值得注意的是，与单次暴露相比，PVC 微塑料和镉的相互作用导致药物代谢和寿命调节相关基因的差异表达。这表明这两种压力源之间的相互作用可能对贻贝健康产生放大的影响。总体而言，这种全面的转录组分析为了解环境中 PVC 微塑料和镉的适应性和有害反应提供了宝贵的见解。