Sophorose is the most effective inducer for cellulase production by Trichoderma reesei. Currently, the biosynthesis of sophorose is very inefficient, resulting in that unavailable for cellulase production in industry. In this study, CoGH1A, a multifunctional thermophilic glycoside hydrolase, was employed for sophorose production. Under the optimized conditions, the sophorose yield was 37.86 g/L with a productivity of 9.47 g/L/h which is by far the highest productivity. Meanwhile, the Fe3O4-CS-THP-CoGH1A nanoparticles were constructed to realize the recycling of CoGH1A. After 5 cycles of catalysis, Fe3O4-CS-THP-CoGH1A retained about 83.90 % enzyme activity. Finally, the mixtures of glucose and disaccharides (MGDC) obtained after being catalyzed by CoGH1A was used for cellulase production. As a result, the cellulase productivity achieved 188.38 FPU/L/h in 120 h. These results indicated that sophorose could be efficiently produced from glucose via transglycosylation by CoGH1A, making it possible to be industrially used as the inducer to improving the cellulase productivity.

中文翻译：

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从葡萄糖高效生产槐糖及其在纤维素酶生产中的潜在工业应用


槐糖是里氏木霉产生纤维素酶最有效的诱导剂。目前槐糖的生物合成效率很低，无法用于工业化纤维素酶生产。在本研究中，CoGH1A（一种多功能嗜热糖苷水解酶）被用于槐糖生产。在优化条件下，槐糖产量为37.86 g/L，生产率为9.47 g/L/h，为迄今为止最高的生产率。同时，构建了Fe3O4-CS-THP-CoGH1A纳米粒子，实现了CoGH1A的循环利用。经过5个循环的催化后，Fe3O4-CS-THP-CoGH1A保留了约83.90%的酶活性。最后，经CoGH1A催化得到的葡萄糖和二糖混合物（MGDC）用于纤维素酶生产。结果，120小时内纤维素酶生产率达到188.38 FPU/L/h。这些结果表明槐糖可以通过CoGH1A的转糖基作用有效地从葡萄糖生产槐糖，使得其可以在工业上用作诱导剂以提高纤维素酶的生产率。