An integrated process of sludge in-situ fermentation, biological phosphorus removal and endogenous denitrification (ISFPR-ED) was developed to treat low ratio of chemical oxygen demand to nitrogen (COD/N) wastewater and waste activated sludge (WAS) in a single reactor. Nutrient removal and WAS reduction were achieved due to Tetrasphaera-dominated sludge fermentation provided organic carbon in extending the anaerobic duration. The WAS reduction efficiency, effluent orthophosphate (PO43−-P) and total inorganic nitrogen reached 28.1 %, less than 0.4 and 7.2 mg/L, respectively. While organic carbon was reduced by 67 %. Tetrasphaera, conventional polyphosphate accumulating organisms (PAOs) stored glycogen, amino acids, and PHA for nutrient removal. Excess energy from fermentation enhanced anaerobic PO43−-P uptake by Tetrasphaera. Tetrasphaera was the dominant PO43−-P removal and fermentation bacteria, working synergistically with conventional PAOs and fermenting microorganisms. This integrated process improves nutrient removal efficiency and reduces operating costs for carbon addition and WAS disposal in wastewater treatment.

中文翻译：

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单反应器内以四球菌为主的废活性污泥原位发酵、生物除磷、内源反硝化一体化工艺处理有限碳源废水


开发了污泥原位发酵、生物除磷和内源反硝化一体化工艺（ISFPR-ED），用于在单反应器中处理低化学需氧量氮比（COD/N）废水和废活性污泥（WAS） 。由于以四球菌为主的污泥发酵提供了有机碳，延长了厌氧持续时间，因此实现了营养物去除和WAS减少。 WAS还原效率、出水正磷酸盐（PO43−P）和总无机氮达到28.1%，分别低于0.4和7.2 mg/L。同时有机碳减少了 67%。 Tetrasphaera 是传统的聚磷酸盐积累生物 (PAO)，储存糖原、氨基酸和 PHA 以去除营养。发酵产生的过量能量增强了四球菌对厌氧 PO43−P 的吸收。 Tetrasphaera 是主要的 PO43−P 去除和发酵细菌，与传统 PAO 和发酵微生物协同作用。这种集成工艺提高了营养物去除效率，并降低了废水处理中碳添加和 WAS 处置的运营成本。