Methods to measure chromatin contacts at genomic regions bound by histone modifications or proteins are important tools to investigate chromatin organization. However, such methods do not capture the possible involvement of other epigenomic features such as G-quadruplex DNA secondary structures (G4s). To bridge this gap, we introduce ViCAR (viewpoint HiCAR), for the direct antibody-based capture of chromatin interactions at folded G4s. Through ViCAR, we showcase the first G4-3D interaction landscape. Using histone marks, we also demonstrate how ViCAR improves on earlier approaches yielding increased signal-to-noise. ViCAR is a practical and powerful tool to explore epigenetic marks and 3D genome interactomes.

中文翻译：

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通过 ViCAR 改进表观遗传特征和 3D 染色质结构的同步映射


测量组蛋白修饰或蛋白质结合的基因组区域染色质接触的方法是研究染色质组织的重要工具。然而，此类方法并未捕获其他表观基因组特征（例如 G-四链体 DNA 二级结构 (G4s)）的可能参与。为了弥补这一差距，我们引入了 ViCAR（观点 HiCAR），用于基于抗体直接捕获折叠 G4 处的染色质相互作用。通过 ViCAR，我们展示了第一个 G4-3D 交互景观。使用组蛋白标记，我们还展示了 ViCAR 如何改进早期方法，从而提高信噪比。 ViCAR 是探索表观遗传标记和 3D 基因组相互作用组的实用而强大的工具。