Chloroform is a prevalent toxic environmental pollutant in urban settings, posing risks to human health through exposure via various mediums such as air and tap water. The gut microbiota plays a pivotal role in maintaining host health. However, there is a paucity of research elucidating the impact of chloroform exposure on the gut microbiota. In this investigation, 18 SPF Kunming female mice were stratified into three groups (n = 6) and subjected to oral gavage with chloroform doses equivalent to 0, 50, and 150 mg/kg of body weight over 30 days. Our findings demonstrate that subchronic chloroform exposure significantly perturbs hematological parameters in mice and induces histopathological alterations in cecal tissues, consequently engendering marked disparities in the functional composition of cecal microbiota and metabolic equilibrium of cecal contents. Ultimately, our investigation revealed a statistically robust correlation, exhibiting a high degree of significance, between the intestinal microbiome composition and the metabolites that were differentially expressed consequent to chloroform exposure.

中文翻译：

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亚慢性氯仿暴露会导致小鼠肠道损伤并诱发肠道微生物群破坏和代谢失调


氯仿是城市环境中普遍存在的有毒环境污染物，通过空气和自​​来水等各种介质接触，对人类健康构成风险。肠道微生物群在维持宿主健康方面发挥着关键作用。然而，很少有研究阐明氯仿暴露对肠道微生物群的影响。在本研究中，将 18 只 SPF 昆明雌性小鼠分为三组（n = 6），并在 30 天内接受相当于 0、50 和 150 mg/kg 体重的氯仿剂量口服灌胃。我们的研究结果表明，亚慢性氯仿暴露显着扰乱小鼠的血液学参数，并诱导盲肠组织的组织病理学改变，从而导致盲肠微生物群的功能组成和盲肠内容物的代谢平衡显着差异。最终，我们的研究揭示了肠道微生物组组成与氯仿暴露后差异表达的代谢物之间存在统计上稳健的相关性，表现出高度显着性。