The stink bugs Edessa meditabunda, Piezodorus guildinii, and Diceraeus furcatus (Hemiptera: Pentatomidae) are major pests in the Argentinean core area of soybean production. A detailed molecular genetics comprehension of how these insects perceive odorants and respond to semiochemicals and how they detoxify chemical pesticides and plant compounds are essential to improve their management strategies. We first assembled and compared the transcriptomes from E. meditabunda, P. guildinii, and D. furcatus. Regarding sequence similarity, P. guildinii and D. furcatus are closer to each other than E. meditabunda. Then, we characterized the multigene families of odorant binding proteins (OBPs) and cytochrome P450 monooxygenases (CYP). A total of 29, 38, and 39 unigenes encoding for OBP were obtained in E. meditabunda, P. guildinii, and D. furcatus, respectively, divided into classical OBPs and plus-C OBPs. A total of 72, 63, and 76 unigenes encoding for CYP were found in E. meditabunda, P. guildinii, and D. furcatus, respectively, which were further classified into 24 families and 47 subfamilies. On the other hand, we performed for the first time RNA interference in vivo by dsRNA injection in E. meditabunda, suggesting that this molecular tool can be exploited in future physiological and functional studies in this species.

臭虫Edessa meditabunda 、 Piezodorus guildinii和Diceraeusfurcatus （半翅目：蝽科）是阿根廷大豆生产核心区的主要害虫。对这些昆虫如何感知气味、对化学信息素作出反应以及它们如何解毒化学农药和植物化合物的详细分子遗传学理解对于改善其管理策略至关重要。我们首先组装并比较了E.meditabunda 、 P.guildinii和D.furcatus的转录组。关于序列相似性， P. guildinii和D.furcatus比E. meditabunda 更接近。然后，我们对气味结合蛋白 (OBP) 和细胞色素 P450 单加氧酶 (CYP) 的多基因家族进行了表征。在E. meditabunda 、 P. guildinii和D.furcatus中分别获得了 29、38 和 39 个编码 OBP 的 unigenes，分为经典 OBP 和 plus-C OBP。在E. meditabunda 、 P. guildinii和D.furcatus中分别发现了 72、63 和 76 个编码 CYP 的 unigenes，分别分为 24 个科和 47 个亚科。另一方面，我们首次通过在E. meditabunda中注射 dsRNA 进行体内 RNA 干扰，表明这种分子工具可用于该物种未来的生理和功能研究。