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SERS-Based Non-Enzymatic Detection of Uric Acid Using Au/CeO2 Nanorods
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2024-09-02 , DOI: 10.1021/acsanm.4c03844 Yifei Li 1 , Peng Li 1 , Mengyao Qi 1 , Xia Zhang 1 , Jun Meng 2
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2024-09-02 , DOI: 10.1021/acsanm.4c03844 Yifei Li 1 , Peng Li 1 , Mengyao Qi 1 , Xia Zhang 1 , Jun Meng 2
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Rapid and sensitive detection of uric acid (UA) has broad application prospects in biosensing and biomedical applications. Based on the surface-enhanced Raman scattering (SERS) system, Au/cerium oxide (CeO2) nanorods with excellent catalytic performance and high SERS activity were developed for highly sensitive detection of UA. Uniformly dispersed rod-like CeO2 was prepared by hydrothermal synthesis. Then, Au NPs were decorated on CeO2 by a simple in situ growth strategy. The concentrations of Ce3+ ions and oxygen vacancies were increased by increasing the number of Au NPs to obtain the optimal modification ratio. Moreover, Au NPs as active SERS substrates further significantly improve the detection sensitivity. Benefiting from the excellent performance, the Au/CeO2 nanorods were combined with 3,3′,5,5′-tetramethylbenzidine (TMB) and H2O2 to explore the valence change of Ce and the internal catalytic mechanism of reactive oxygen species generation in the reaction. Under optimized conditions, the SERS system for detection of UA was constructed for enzyme-free, with a wide range and low limit of detection of 3.29 × 10–10 M. Based on the strong peroxidase-like activity of Au/CeO2 nanorods under acidic conditions, it was successfully applied to the detection of UA in human urine samples. Therefore, this study provides proof that Au NPs enhance the peroxidase-like activity of Au/CeO2 nanorods for SERS detection of UA.
中文翻译：
使用 Au/CeO2 纳米棒进行基于 SERS 的尿酸非酶检测
快速、灵敏的尿酸（UA）检测在生物传感和生物医学应用中具有广阔的应用前景。基于表面增强拉曼散射（SERS）系统，开发了具有优异催化性能和高SERS活性的Au/氧化铈（CeO 2 ）纳米棒，用于UA的高灵敏检测。采用水热合成法制备了均匀分散的棒状CeO 2 。然后，通过简单的原位生长策略将Au NPs 装饰在CeO 2上。通过增加Au NPs的数量来增加Ce 3+离子和氧空位的浓度，以获得最佳的修饰比例。此外，Au NPs作为活性SERS底物进一步显着提高了检测灵敏度。受益于优异的性能，Au/CeO 2纳米棒与3,3′,5,5′-四甲基联苯胺(TMB)和H 2 O 2结合，探索Ce的价态变化和活性氧的内催化机制反应中生成。在优化条件下，构建了无酶检测UA的SERS系统，检测范围宽，检测下限为3.29 × 10 –10 M。酸性条件下，成功应用于人尿样中UA的检测。因此，本研究证明Au NPs增强了Au/CeO 2纳米棒的类过氧化物酶活性，用于UA的SERS检测。
更新日期：2024-09-02
中文翻译：
使用 Au/CeO2 纳米棒进行基于 SERS 的尿酸非酶检测
快速、灵敏的尿酸（UA）检测在生物传感和生物医学应用中具有广阔的应用前景。基于表面增强拉曼散射（SERS）系统，开发了具有优异催化性能和高SERS活性的Au/氧化铈（CeO 2 ）纳米棒，用于UA的高灵敏检测。采用水热合成法制备了均匀分散的棒状CeO 2 。然后，通过简单的原位生长策略将Au NPs 装饰在CeO 2上。通过增加Au NPs的数量来增加Ce 3+离子和氧空位的浓度，以获得最佳的修饰比例。此外，Au NPs作为活性SERS底物进一步显着提高了检测灵敏度。受益于优异的性能，Au/CeO 2纳米棒与3,3′,5,5′-四甲基联苯胺(TMB)和H 2 O 2结合，探索Ce的价态变化和活性氧的内催化机制反应中生成。在优化条件下，构建了无酶检测UA的SERS系统，检测范围宽，检测下限为3.29 × 10 –10 M。酸性条件下，成功应用于人尿样中UA的检测。因此，本研究证明Au NPs增强了Au/CeO 2纳米棒的类过氧化物酶活性，用于UA的SERS检测。
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