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Prenylated Flavonoids Isolated from the Root of Sophora flavescens as Potent Antifungal Agents against Botrytis cinerea
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-08-28 , DOI: 10.1021/acs.jafc.4c04209 Jun-Xia An 1, 2 , Rui Wang 3 , An-Ping Li 4 , Wen Zhang 2 , Zhibiao Nan 1 , Wei-Qi Jiang 2 , Shao-Yong Zhang 5 , Zhi-Jun Zhang 2 , Xiong-Fei Luo 2 , Hong-Jie Liang 2 , Ying-Qian Liu 1, 2, 5
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-08-28 , DOI: 10.1021/acs.jafc.4c04209 Jun-Xia An 1, 2 , Rui Wang 3 , An-Ping Li 4 , Wen Zhang 2 , Zhibiao Nan 1 , Wei-Qi Jiang 2 , Shao-Yong Zhang 5 , Zhi-Jun Zhang 2 , Xiong-Fei Luo 2 , Hong-Jie Liang 2 , Ying-Qian Liu 1, 2, 5
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Sophora flavescens, a traditional Chinese herb, produces a wide range of secondary metabolites with a broad spectrum of biological activities. In this study, we isolated six isopentenyl flavonoids (1–6) from the roots of S. flavescens and evaluated their activities against phytopathogenic fungi. In vitro activities showed that kurarinone and sophoraflavanone G displayed broad spectrum and superior activities, among which sophoraflavanone G displayed excellent activity against tested fungi, with EC50 values ranging from 4.76 to 13.94 μg/mL. Notably, kurarinone was easily purified and showed potential activity against Rhizoctonia solani, Botrytis cinerea, and Fusarium graminearum with EC50 values of 16.12, 16.55, and 16.99 μg/mL, respectively. Consequently, we initially investigated the mechanism of kurarinone against B. cinerea. It was found that kurarinone disrupted cell wall components, impaired cell membrane integrity, increased cell membrane permeability, and affected cellular energy metabolism, thereby exerting its effect against B. cinerea. Therefore, kurarinone is expected to be a potential candidate for the development of plant fungicides.
中文翻译：
从苦参根中分离出的异戊二烯化黄酮类化合物作为有效的抗灰霉病真菌剂
苦参是一种传统中药材，可产生多种具有广谱生物活性的次生代谢产物。在这项研究中，我们从苦参根中分离出六种异戊烯基黄酮类化合物(1-6) ，并评估了它们对抗植物病原真菌的活性。体外活性表明，苦参酮和槐花黄酮G具有广谱和优异的活性，其中槐花黄酮G对受试真菌具有优异的活性，EC 50值在4.76~13.94 μg/mL之间。值得注意的是，苦参酮易于纯化，并且对立枯丝核菌、灰葡萄孢和禾谷镰刀菌表现出潜在活性，EC 50值分别为 16.12、16.55 和 16.99 μg/mL。因此，我们初步研究了苦参酮对抗灰霉病的机制。研究发现，苦参酮破坏细胞壁成分，损害细胞膜完整性，增加细胞膜通透性，影响细胞能量代谢，从而发挥对抗灰霉病的作用。因此，苦参酮有望成为开发植物杀菌剂的潜在候选者。
更新日期：2024-09-01
中文翻译：
从苦参根中分离出的异戊二烯化黄酮类化合物作为有效的抗灰霉病真菌剂
苦参是一种传统中药材，可产生多种具有广谱生物活性的次生代谢产物。在这项研究中，我们从苦参根中分离出六种异戊烯基黄酮类化合物(1-6) ，并评估了它们对抗植物病原真菌的活性。体外活性表明，苦参酮和槐花黄酮G具有广谱和优异的活性，其中槐花黄酮G对受试真菌具有优异的活性，EC 50值在4.76~13.94 μg/mL之间。值得注意的是，苦参酮易于纯化，并且对立枯丝核菌、灰葡萄孢和禾谷镰刀菌表现出潜在活性，EC 50值分别为 16.12、16.55 和 16.99 μg/mL。因此，我们初步研究了苦参酮对抗灰霉病的机制。研究发现，苦参酮破坏细胞壁成分，损害细胞膜完整性，增加细胞膜通透性，影响细胞能量代谢，从而发挥对抗灰霉病的作用。因此，苦参酮有望成为开发植物杀菌剂的潜在候选者。
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