To investigate the role of nitric oxide (NO) in oral tissue damage and periodontal diseases, this study designed and synthesized a novel near-infrared fluorescent probe, BPTQ. The probe BPTQ can react with NO through its amino group to form N-nitroso compounds, which induces a highly selective and sensitive fluorescence "turn-on" effect. The probe exhibited a detection limit of 15 nM and responded rapidly within 30 s. Analysis of saliva samples from 36 healthy individuals and those with oral diseases demonstrated a direct link between NO levels and the occurrence of oral diseases. Cell experiments further confirmed the low toxicity of the probe and its ability to effectively monitor endogenous NO in cells. Therefore, the BPTQ fluorescent probe developed in this study represents a promising new tool for monitoring and assessing the progression of oral diseases.

中文翻译：

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用于唾液和细胞环境中一氧化氮检测和成像的增强型近红外荧光探针


为了研究一氧化氮（NO）在口腔组织损伤和牙周疾病中的作用，本研究设计并合成了一种新型近红外荧光探针BPTQ。探针BPTQ可以通过其氨基与NO反应形成N-亚硝基化合物，从而诱导高选择性和灵敏的荧光“开启”效应。该探针的检测限为 15 nM，并在 30 秒内快速响应。对 36 名健康人和口腔疾病患者唾液样本的分析表明，NO 水平与口腔疾病的发生之间存在直接联系。细胞实验进一步证实了该探针的低毒性及其能够有效监测细胞内源性NO的能力。因此，本研究开发的 BPTQ 荧光探针代表了一种有前途的监测和评估口腔疾病进展的新工具。