Methylpyridines are a class of highly toxic pyridine derivatives. In this study, a novel degrading bacterium was isolated for 3-methylpyridine (3-MP) degradation (Gordonia rubripertincta ZJJ, GenBank accession NO. OP430847.1; CCTCC M 2022975). The maximum specific degradation rate, half-saturation constant and inhibition constant were fitted to be 0.48 h−1, 88.3 mg L−1 and 924.0 mg L−1, respectively. During 3-MP biodegradation, the lost total organic carbon was transformed into CO2 (67.4 %) and biomass (32.6 %), and ammonia nitrogen was almost the sole inorganic species with a conversion rate of 36.3 %. Three metabolic pathways were possibly involved in 3-MP degradation: I) methyl oxidation followed by ring hydroxylation and hydrogenation; II) rupture of C=C and C–N bonds after ring reduction; III) initial ring hydroxylation. The study not only provides a novel strain for the high-efficient degradation of 3-MP, but also contributes to an in-depth understanding of 3-MP biotransformation.

中文翻译：

---

分离菌株 Gordonia rubripertincta ZJJ 对 3-甲基吡啶的生物降解


甲基吡啶是一类剧毒的吡啶衍生物。在本研究中，分离出一种用于 3-甲基吡啶 (3-MP) 降解的新型降解细菌（Gordonia rubripertincta ZJJ，GenBank 登录号 OP430847.1；CCTCC M 2022975）。最大比降解率、半饱和常数和抑制常数分别拟合为0.48 h−1、88.3 mg L−1 和924.0 mg L−1。在3-MP生物降解过程中，损失的总有机碳转化为CO2（67.4%）和生物质（32.6%），氨氮几乎是唯一的无机物，转化率为36.3%。 3-MP降解可能涉及三种代谢途径：I)甲基氧化，随后环羟基化和氢化； II)环还原后C=C和C-N键断裂； III)初始环羟基化。该研究不仅为3-MP的高效降解提供了新的菌株，也有助于深入了解3-MP的生物转化。