Lignocellulosic fermentable sugars have been attracting increasing attention due to the developments of synthetic biology, yet the heterogeneity of the raw material results in high pretreatment intensities and the generation of numerous fermentation inhibitors. In this study, the rolling-disc milling texture-sorting was established for raw material to mitigate inhibitors generation at its source, specifically targeting the variances in mechanical strength among corn straw tissues. Through the texture-sorting of raw corn straw, the low modulus of elasticity tissues in corn straw were crushed into soft-texture sorts and the high-strength tissues were retained as hard-texture sorts. The results showed that the steam explosion pretreatment intensity required for soft-texture sorts decreased by 77.34 %, from 1125.96 (180 °C, 5 min) to 255.10 (160 °C, 5 min), compared to the hard-texture sorts. The concentrations of inhibitors in the enzymatic hydrolysate of soft-texture sorts decreased by 55.10 %, from 8.13 g/L/(100 g/L total sugars) to 3.65 g/L/(100 g/L total sugars), indicating an improved suitability for enzymatic hydrolysate preparation. Therefore, the rolling-disc milling texture-sorting method was proposed to provide a new approach for the preparation of lignocellulosic fermentable sugars.

中文翻译：

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原秸秆滚盘磨碎质地分选有利于降低汽爆强度


随着合成生物学的发展，木质纤维素发酵糖越来越受到人们的关注，但原料的异质性导致预处理强度高，并产生大量发酵抑制剂。在本研究中，针对原材料建立了滚盘铣削纹理分类，以从源头减少抑制剂的产生，特别针对玉米秸秆组织之间机械强度的差异。通过对原玉米秸秆进行质地分选，将玉米秸秆中弹性模量较低的组织粉碎成质地较软的组织，保留高强度组织作为质地较硬的组织。结果表明，与硬质品种相比，软质品种所需的汽爆预处理强度从1125.96（180℃，5分钟）降低到255.10（160℃，5分钟），降低了77.34%。软质品种酶解液中抑制剂浓度下降了55.10%，从8.13 g/L/(100 g/L总糖)降至3.65 g/L/(100 g/L总糖)，表明软质品种酶解液中抑制剂的浓度下降了55.10%，表明酶解液中抑制剂的浓度有所提高。酶解产物制备的适用性。因此，提出滚盘研磨质构分选方法，为木质纤维素发酵糖的制备提供新途径。