Valorization of food waste into high value-added glycerol was designed and prepared via directional green transformation using multi-enzymolysis saccharification coupled facultative fermentation by . The optimal conditions for the first step of multienzyme hydrolysis and the second step of facultative fermentation were 55 °C and pH 5.0 for 6 h, 30 °C and pH 7.0 for 70 h, respectively. The glycerol yield efficiency and kinetic rate constant were 73.6 % (/) and 0.681 h for dry component of food waste, while the other 27.4 % was utilized as the growth energy substrate. The key points of transformation were the synergistic directed enzymolysis to produce 3 % glycerol and 95 % reducing sugars. Furthermore, the reducing sugars were converted into glycerol by switch the metabolic pathway from ethanol into glycerol through the quantitative expression of functional and genes activated GPD and GPP enzymatic activity and synchronous inhibition of gene for glycerol production with NaSO regulation. The economic income was 135.52 USD/ton for converting 80 % water content of kitchen waste into glycerol. This finding provided an economic value-added approach to produce high-value glycerol for cost-effective recycling of food waste using commercial yeast.

中文翻译：

---

利用酿酒酵母通过两步分化酶解和兼性发酵将食物垃圾绿色定向转化为甘油：性能评估和机制


通过多酶解糖化耦合兼性发酵的定向绿色转化，设计和制备了将食物垃圾增值为高附加值甘油。第一步多酶水解和第二步兼性发酵的最佳条件分别为55℃、pH 5.0、6 h；30℃、pH 7.0、70 h。食品垃圾干成分的甘油产率和动力学速率常数分别为73.6%（/）和0.681 h，其余27.4%用作生长能量底物。转化的关键是协同定向酶解生产3%甘油和95%还原糖。此外，通过功能性和基因激活的GPD和GPP酶活性的定量表达以及通过NaSO调节同步抑制甘油生产基因，将代谢途径从乙醇转换为甘油，从而将还原糖转化为甘油。将80%含水量的餐厨垃圾转化为甘油，经济收入为135.52美元/吨。这一发现提供了一种经济增值方法，可以使用商业酵母生产高价值甘油，从而经济高效地回收食物垃圾。