This study investigated the impact of different ratios of soluble to insoluble dietary fiber (SDF:IDF) formulations by sugar beet pulp (SBP) supplementation on piglet growth performance, nutrient digestibility, immune function, intestinal morphology, intestinal microbiota and intestinal health. A total of 60 crossbred piglets (Duroc × [Landrace × Yorkshire]) at 40 d old with body weight of 10.0 ± 0.3 kg were randomly assigned to 5 treatments with 6 replicates per treatment and 2 piglets per replicate in a 21-d trial. The dietary treatments included a corn-soybean meal diet (0% SBP supplementation; CON), and diets supplemented with 2%, 4%, 6%, and 8% SBP, representing different SDF:IDF ratios at 10.16%, 13.53%, 16.79%, 19.86%, and 24.81%, respectively. The results indicated that the 8% SBP treatment had a negative effect on feed-to-gain ratio (linear, P = 0.009) compared with the CON treatment (P = 0.021). The apparent total tract digestibility (ATTD) of crude protein was lower in treatments supplemented with SBP (P = 0.002) and showed a linear decrease (P = 0.001), while the ATTD of IDF showed a linear increase (P = 0.037) in four SBP treatments compared to the CON treatment. The 4% SBP treatment increased serum concentrations of triglyceride (quadratic, P = 0.019) and K (linear, P = 0.037), and decreased alanine transaminase concentration (quadratic, P = 0.015) compared with the CON treatment. The concentrations of Cit, Cys, Ile, Leu, Orn, Arg, taurine, urea, 1-methylhistidine, α-aminoadipic acid, α-aminobutyric acid and cystathionine in the 4% SBP treatment were highest among all treatments (P < 0.05). The serum concentrations of interleukin-6, interleukin-8, interleukin-10, transforming growth factor-β, and tumor necrosis factor-α in the 6% SBP treatment were higher than those in the CON treatment (P < 0.05), which also increased mucin-2 and G protein-coupled receptor 41 mRNA expression (P < 0.05) in colonic mucosa compared with the CON treatment and improved the intestinal barrier function. Diets containing more than 19.86% SDF:IDF could impair the intestinal health in piglets when SBP was used as the SDF source. Supplementing nursery piglet diets with 16.79% to 19.86% SDF:IDF is recommended for improving intestinal barrier function, increasing short-chain fatty acids concentrations, and improving intestinal microbiota composition.

中文翻译：

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不同可溶性膳食纤维与不溶性膳食纤维配比对仔猪生长性能和肠道健康的影响


本研究调查了甜菜浆 （SBP） 补充剂不同比例的可溶性与不溶性膳食纤维 （SDF：IDF） 配方对仔猪生长性能、营养物质消化率、免疫功能、肠道形态、肠道微生物群和肠道健康的影响。在 21 天试验中，将 60 头 40 d 龄、体重为 10.0 ± 0.3 kg 的杂交仔猪 （Duroc × [Landrace × Yorkshire]）随机分配到 5 个处理中，每个处理 6 个重复，每个重复 2 头仔猪。饮食处理包括玉米豆粕饮食（0% SBP 补充剂;CON） 和添加 2% 、 4% 、 6% 和 8% SBP 的饮食，代表不同的 SDF：IDF 比率分别为 10.16% 、 13.53% 、 16.79% 、 19.86% 和 24.81%。结果表明，与 CON 处理 （P = 0.021） 相比，8% SBP 处理对饲料增重比 （线性，P = 0.009） 有负面影响。在添加 SBP 的处理中，粗蛋白的表观总消化率 （ATTD） 较低 （P = 0.002） 并呈线性下降 （P = 0.001），而 IDF 的 ATTD 在四种 SBP 处理中呈线性增加 （P = 0.037） 与 CON 处理相比。与 CON 处理相比，4% SBP 处理增加了血清甘油三酯浓度 （二次方，P = 0.019） 和 K （线性，P = 0.037），降低了丙氨酸转氨酶浓度 （二次方，P = 0.015）。4% SBP 处理中 Cit、Cys、Ile、Leu、Orn、Arg、牛磺酸、尿素、1-甲基组氨酸、α-氨基己二酸、α-氨基丁酸和胱硫醚的浓度在所有处理中最高（P < 0.05）。 6% SBP 处理血清白细胞介素-6、白细胞介素-8、白细胞介素-10、转化生长因子-β和肿瘤坏死因子-α浓度高于 CON 处理（P < 0.05），与CON处理相比，结肠粘膜粘蛋白-2 和 G 蛋白偶联受体 41 mRNA 表达 （P < 0.05） 并改善肠道屏障功能。当 SBP 作为 SDF 来源时，SDF：IDF 含量超过 19.86% 的日粮会损害仔猪的肠道健康。建议在保育仔猪日粮中补充 16.79% 至 19.86% SDF：IDF，以改善肠道屏障功能，增加短链脂肪酸浓度，并改善肠道微生物群组成。