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Polystyrene microplastics induce molecular toxicity in Simocephalus vetulus: A transcriptome and intestinal microorganism analysis
Aquatic Toxicology ( IF 4.1 ) Pub Date : 2024-08-08 , DOI: 10.1016/j.aquatox.2024.107046
Chenxi Zhu 1 , Hui Zhou 2 , Mengyu Bao 3 , Shengkai Tang 4 , Xiankun Gu 4 , Mingming Han 4 , Peng Li 2 , Qichen Jiang 4
Aquatic Toxicology ( IF 4.1 ) Pub Date : 2024-08-08 , DOI: 10.1016/j.aquatox.2024.107046
Chenxi Zhu 1 , Hui Zhou 2 , Mengyu Bao 3 , Shengkai Tang 4 , Xiankun Gu 4 , Mingming Han 4 , Peng Li 2 , Qichen Jiang 4
Affiliation
The global prevalence and accumulation of plastic waste is leading to pollution levels that cause significant damage to ecosystems and ecological security. Exposure to two concentrations (1 and 5 mg/L) of 500 nm polystyrene (PS)-nanoplastics (NPs) for 14 d was evaluated in using transcriptome and 16 s rRNA sequencing analyses. PS-NP exposure resulted in stress-induced antioxidant defense, disturbed energy metabolism, and affected the FoxO signaling pathway, causing neurotoxicity. The expression of Cyclin D1 (CCND), glucose-6-phosphatase (G6PC) and phosphoenolpyruvate carboxykinase (PCK) genes was decreased compared to the control, whereas the expression of caspase3 (CASP3), caspase7 (CASP7), Superoxide dismutase (SOD), Heat shock protein 70 (HSP70), MPV17, and Glutathione S-transferase (GST) genes was increased, thus, suggesting that NP ingestion triggered oxidative stress and disrupted energy metabolism.. PS-NPs were present in the digestive tract of after 14 days of exposure. In addition, the abundance of the Proteobacteria and opportunistic pathogens was elevated after PS-NPs exposure. The diversity and homeostasis of the gut microbiota were disrupted and the stability of intestinal barrier function was impaired. Multiomic analyses highlighted the molecular toxicity and microbial changes in after exposure to NPs, providing an overview of how plastic pollution affects freshwater organisms and ecosystems.
中文翻译:
聚苯乙烯微塑料在 Simocephalus vetulus 中诱导分子毒性:转录组和肠道微生物分析
全球塑料废物的普遍存在和积累正在导致污染水平对生态系统和生态安全造成重大损害。使用转录组和 16 s rRNA 测序分析评估暴露于两种浓度(1 和 5 mg/L)的 500 nm 聚苯乙烯 (PS)-纳米塑料 (NP) 14 天。 PS-NP 暴露会导致应激诱导的抗氧化防御,扰乱能量代谢,并影响 FoxO 信号通路,导致神经毒性。与对照相比,Cyclin D1 (CCND)、葡萄糖-6-磷酸酶 (G6PC) 和磷酸烯醇丙酮酸羧激酶 (PCK) 基因的表达量减少,而 caspase3 (CASP3)、caspase7 (CASP7)、超氧化物歧化酶 (SOD) 的表达量降低,热休克蛋白 70 (HSP70)、MPV17 和谷胱甘肽 S-转移酶 (GST) 基因增加,因此表明 NP 摄入引发氧化应激并扰乱能量代谢。14 岁后消化道中存在 PS-NP暴露天数。此外,接触 PS-NP 后,变形菌和机会性病原体的丰度有所升高。肠道菌群的多样性和稳态被破坏,肠道屏障功能的稳定性受损。多组学分析强调了接触纳米粒子后的分子毒性和微生物变化,概述了塑料污染如何影响淡水生物和生态系统。
更新日期:2024-08-08
中文翻译:
聚苯乙烯微塑料在 Simocephalus vetulus 中诱导分子毒性:转录组和肠道微生物分析
全球塑料废物的普遍存在和积累正在导致污染水平对生态系统和生态安全造成重大损害。使用转录组和 16 s rRNA 测序分析评估暴露于两种浓度(1 和 5 mg/L)的 500 nm 聚苯乙烯 (PS)-纳米塑料 (NP) 14 天。 PS-NP 暴露会导致应激诱导的抗氧化防御,扰乱能量代谢,并影响 FoxO 信号通路,导致神经毒性。与对照相比,Cyclin D1 (CCND)、葡萄糖-6-磷酸酶 (G6PC) 和磷酸烯醇丙酮酸羧激酶 (PCK) 基因的表达量减少,而 caspase3 (CASP3)、caspase7 (CASP7)、超氧化物歧化酶 (SOD) 的表达量降低,热休克蛋白 70 (HSP70)、MPV17 和谷胱甘肽 S-转移酶 (GST) 基因增加,因此表明 NP 摄入引发氧化应激并扰乱能量代谢。14 岁后消化道中存在 PS-NP暴露天数。此外,接触 PS-NP 后,变形菌和机会性病原体的丰度有所升高。肠道菌群的多样性和稳态被破坏,肠道屏障功能的稳定性受损。多组学分析强调了接触纳米粒子后的分子毒性和微生物变化,概述了塑料污染如何影响淡水生物和生态系统。