Capillary electrochromatography (CEC) stationary phases have always been the focus of attention. The selection of excellent stationary phases are the key to realize separate of different compounds. Hydrogen-bonded organic frameworks (HOFs) are porous materials connected by hydrogen bonds between molecules, which have the advantages of renewable, high specific surface area and mild synthesis conditions. At present, HOFs are used in gas adsorption and storage, catalysis and drug delivery. Because of its unique advantages, HOFs have a bright future as CEC stationary phases. Using melamine (MA) and -tetra (-carboxylphenyl)pyrene (HTBAPy) as reaction monomers, a HOFs named MA/PFC-1 was synthesized by solvent evaporation at room temperature. The inner wall of the capillary column was coated with MA/PFC-1 by chemical bonding. Sulfonamides were used as the target analytes. The effects of pH, phosphate buffer solution concentration, organic additive content and applied voltage on sulfonamides separation were investigated. The MA/PFC-1-coated capillary column had good resolution (>1.5) and reproducibility. The intra-day, inter-day, column-to-column, and inter-batch precision of the retention times were 0.03%–0.09%, 0.04%–0.09%, 0.03%–0.14% and 0.06%–0.09%, respectively. The intra-day, inter-day, column-to-column, and inter-batch precision of the peak areas were 0.11%–0.25%, 0.13%–0.20%, 0.12%–0.15% and 0.08%–0.15%, respectively. The MA/PFC-1-coated capillary column was run 150 consecutive times, and the results showed no noticeable change, which proved that this method had good stability. This work applied HOFs to CEC. The results show the that MA/PFC-1-coated capillary column has good separation performance. The MA/PFC-1-coated capillary column has been successfully applied to the determination of sulfamethoxazole in tablets, which has practical application value. To open up the application of HOFs in CEC and provide a new idea for developing new CEC stationary phases.

中文翻译：

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氢键有机骨架作为开管毛细管电色谱固定相


毛细管电色谱（CEC）固定相一直是人们关注的焦点。选择优良的固定相是实现不同化合物分离的关键。氢键有机骨架材料（HOFs）是分子间通过氢键连接的多孔材料，具有可再生、高比表面积和合成条件温和等优点。目前，HOFs应用于气体吸附和储存、催化和药物输送等领域。由于其独特的优势，HOF作为CEC固定相有着光明的前景。以三聚氰胺（MA）和四（-羧基苯基）芘（HTBAPy）为反应单体，通过室温蒸发溶剂合成了HOFs，命名为MA/PFC-1。毛细管柱内壁通过化学键合涂覆有MA/PFC-1。使用磺酰胺作为目标分析物。考察了pH、磷酸盐缓冲液浓度、有机添加剂含量和外加电压对磺胺类药物分离的影响。 MA/PFC-1 涂层毛细管柱具有良好的分辨率 (>1.5) 和重现性。保留时间的日内、日间、柱间和批次间精密度分别为 0.03%–0.09%、0.04%–0.09%、0.03%–0.14% 和 0.06%–0.09% 。峰面积的日内、日间、柱间和批间精密度分别为 0.11%–0.25%、0.13%–0.20%、0.12%–0.15% 和 0.08%–0.15% 。 MA/PFC-1涂层毛细管柱连续运行150次，结果无明显变化，证明该方法具有良好的稳定性。这项工作将 HOF 应用于 CEC。结果表明MA/PFC-1涂层毛细管柱具有良好的分离性能。 MA/PFC-1涂层毛细管柱已成功应用于片剂中磺胺甲恶唑的测定，具有实际应用价值。开拓HOFs在CEC中的应用，为开发新型CEC固定相提供新思路。