Understanding the pathogenic crystallization of calcium oxalate (CaOx), a major constituent of kidney stones, is crucial in developing effective treatment strategies. In this work, the interactions between lysozyme and CaOx during crystallization have been investigated. Further investigations into the crystal morphology, size, quantity, and nucleation time of the lysozyme have been designed. The results reveal that CaCl could act as an effective precipitant for lysozyme crystallization. The formation process and structure of the distinctive composite CaOx-lysozyme crystal has been investigated. The addition of lysozyme promoted the formation of individual CaOx crystals by preventing the crystal aggregation in the solution. The presence of lysozyme induced a transformation in the CaOx crystals from calcium oxalate monohydrate to calcium oxalate dihydrate. These findings contribute to a better understanding of protein-CaOx interactions and their implications in kidney stone pathogenesis.

中文翻译：

---

结晶过程中蛋白质-草酸钙的相互作用


了解草酸钙 (CaOx)（肾结石的主要成分）的致病结晶对于制定有效的治疗策略至关重要。在这项工作中，研究了结晶过程中溶菌酶和 CaOx 之间的相互作用。进一步研究了溶菌酶的晶体形态、尺寸、数量和成核时间。结果表明，CaCl 可以作为溶菌酶结晶的有效沉淀剂。研究了独特的CaOx-溶菌酶复合晶体的形成过程和结构。溶菌酶的添加通过防止溶液中晶体聚集来促进单个 CaOx 晶体的形成。溶菌酶的存在诱导 CaOx 晶体从一水草酸钙转变为二水草酸钙。这些发现有助于更好地了解蛋白质-CaOx 相互作用及其在肾结石发病机制中的影响。