Engineered Microbial Consortium for De Novo Production of Sclareolide,Journal of Agricultural and Food Chemistry

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Engineered Microbial Consortium for De Novo Production of Sclareolide
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-08-30 , DOI: 10.1021/acs.jafc.4c05506
Dandan Tang ₁ , Xianliang Zheng _{2,

3} , Yushuo Zhao ₁ , Chengsong Zhang ₁ , Cheng Chen ₁ , Yuexing Chen ₁ , Lei Du ₁ , Kun Liu ₁ , Shengying Li _{1,

4}

Affiliation

Sclareolide, a natural product with bioactive and fragrant properties, is not only utilized in the food, healthcare, and cosmetics industries but also serves as a precursor for the production of ambroxide and some bioactive compounds. Currently, there are three primary methods for producing sclareolide: direct extraction from plants, chemical synthesis using sclareol as a precursor, and the biotransformation of sclareol. Here, we established a platform for producing sclareolide through a modular coculture system with Saccharomyces cerevisiae and Cryptococcus albidus ATCC 20918. S. cerevisiae was engineered for de novo sclareol biosynthesis from glucose, while C. albidus enabled the production of sclareolide via sclareol biotransformation. To enhance the supply of sclareol, a recombinant yeast strain was constructed through metabolic engineering to produce 536.2 mg/L of sclareol. Further improvement of the coculture system for sclareolide production was achieved by incorporating Triton X-100 facilitated intermediate permeability, inoculation proportion adjustment, and culture temperature optimization. These refinements culminated in a sclareolide yield of 626.3 mg/L. This study presents a novel streamlined and efficient approach for sclareolide preparation, showcasing the potential of the microbial consortium in sustainable bioproduction.

中文翻译：

用于从头生产香紫苏内酯的工程微生物联盟

香紫苏内酯是一种具有生物活性和芳香特性的天然产物，不仅用于食品、保健和化妆品行业，而且还可以作为生产氨溴索和一些生物活性化合物的前体。目前，香紫苏内酯的生产方法主要有三种：从植物中直接提取、以香紫苏醇为前体的化学合成法以及香紫苏醇的生物转化法。在这里，我们建立了一个通过与酿酒酵母和浅白隐球菌ATCC 20918 的模块化共培养系统生产香紫苏内酯的平台。酿酒酵母被设计用于从葡萄糖中从头生物合成香紫苏醇，而浅白酵母则能够通过香紫苏醇生物转化生产香紫苏内酯。为了提高香紫苏醇的供应，通过代谢工程构建了重组酵母菌株，产量为536.2 mg/L香紫苏醇。通过加入 Triton X-100 促进中间渗透性、接种比例调整和培养温度优化，进一步改进了香紫苏内酯生产的共培养系统。这些改进最终使香紫苏内酯的产量达到 626.3 mg/L。这项研究提出了一种新的简化且高效的香紫苏内酯制备方法，展示了微生物群落在可持续生物生产中的潜力。

更新日期：2024-09-01

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