The combination of micron zero-valent iron (mZVI) and microorganisms is an effective method for trichloroethylene (TCE) degradation, but electron transfer efficiency needs improvement. A new chem-bio hybrid process using a composite material (S-ZVI@biochar) was developed, consisting of sulfurized mZVI and biochar as a chemical remover, and Shewanella oneidensis MR-1 and dechlorinating bacteria (DB) as a biological agent for TCE degradation. S-ZVI@biochar showed improved stability, biocompatibility, and TCE removal compared to ZVI and S-ZVI. The hybrid system DB + MR-1 + S-ZVI@biochar exhibited the highest TCE removal efficiency at 96.5% after 30 days, which was 3.7 times higher than that of bare ZVI. The study revealed that the enhanced dechlorination performance was due to improved electron transfer efficiency, adjustment of microbial community structure, and iron recycling. S-ZVI@biochar constructed electron transport channels in the composite system, improving the overall dechlorination capacity. This system shows promise for long-term TCE removal in anaerobic environments.

微米零价铁（mZVI）与微生物的结合是降解三氯乙烯（TCE）的有效方法，但电子传递效率有待提高。开发了一种使用复合材料（S-ZVI@biochar）的新化学-生物混合工艺，其中硫化 mZVI 和生物炭作为化学去除剂，Shewanella oneidensis MR-1 和脱氯细菌（DB）作为 TCE 的生物剂降解。与 ZVI 和 S-ZVI 相比，S-ZVI@biochar 显示出更高的稳定性、生物相容性和 TCE 去除率。混合系统DB + MR-1 + S-ZVI@biochar在30天后表现出最高的TCE去除效率，达到96.5%，是裸ZVI的3.7倍。研究表明，脱氯性能的增强是由于电子传递效率的提高、微生物群落结构的调整和铁的回收利用。 S-ZVI@biochar在复合体系中构建了电子传输通道，提高了整体脱氯能力。该系统有望在厌氧环境中长期去除 TCE。