Study objectives were to evaluate the effects of mitoquinol (MitoQ) on production parameters, gastrointestinal tract (GIT; stomach and small and large intestines) weight, and circulating leukocytes during a 24-h acute heat stress (HS) challenge. Crossbred gilts [n = 32; 49.1 ± 2.4 kg body weight (BW)] were blocked by BW and randomly assigned to 1 of 4 environmental-therapeutic treatments: 1) thermoneutral (TN) control (n = 8; TNCON), 2) TN and MitoQ (n = 8; TNMitoQ), 3) HS control (n = 8; HSCON), or 4) HS and MitoQ (n = 8; HSMitoQ). Pigs were moved into individual pens and allowed to acclimate for 6 d. The study consisted of 2 experimental periods (P). During P1 (2 d), all pigs remained in TN conditions (20.6 ± 1.5 °C) and were fed ad libitum. During P2 (24 h), pigs were fed ad libitum and exposed to either TN or constant HS (37.3 ± 1.3 °C). Mitoquinol (40 mg/d) was orally administered twice daily (0700 and 1800 hours) during P1 and P2. As expected, pigs exposed to HS had increased rectal temperature, skin temperature, and respiration rate (+1.5 °C, +8.7 °C, and +86 bpm, respectively; P < 0.01) compared to their TN counterparts. Compared to TN, HS pigs had decreased feed intake (67%; P < 0.01) and significant BW loss (+1.5 vs. −1.9 kg, respectively; P < 0.01). Total GIT weight was decreased in HS relative to TN pigs (P < 0.01), and this was influenced by decreased luminal contents (2.43 vs. 3.26 kg, respectively; P < 0.01) and reduced empty GIT mass (3.21 vs. 3.48 kg, respectively; P = 0.03). Stomach contents remained similar between TN and HS pigs (P > 0.54) but tended to increase in MitoQ relative to CON pigs (0.90 vs. 0.63 kg, respectively; P = 0.08). Stomach content as a percentage of the previous 24 h feed intake was increased in HS compared to the TN controls (93% vs. 31%; P < 0.01). In contrast, small and large intestinal contents were decreased in HS compared to TN pigs (23% and 49%, respectively; P < 0.01). Liver weight decreased in HS relative to TN pigs (1.15 vs. 1.22 kg, respectively; P = 0.02), and was decreased in MitoQ compared to CON pigs (1.13 vs. 1.24 kg; P < 0.01). Circulating lymphocytes tended to be decreased in HS relative to TN pigs (16%; P = 0.07). In summary, acute HS increased all body temperature indices, negatively influenced animal performance, and differentially altered GIT motility as evidenced by decreased gastric emptying and increased intestinal transit. However, MitoQ supplementation did not appear to ameliorate these effects.

中文翻译：

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米托喹醇在生长后备母猪急性热应激应激应激期间的治疗效果


研究目标是评估丝裂喹醇 （MitoQ） 在 24 小时急性热应激 （HS） 攻击期间对生产参数、胃肠道 （GIT;胃和小肠和大肠） 重量和循环白细胞的影响。杂交后备母猪 [n = 32;49.1 ± 2.4 kg 体重 （BW）] 被 BW 封闭并随机分配到 4 种环境治疗处理中的 1 种：1） 热中性 （TN） 对照 （n = 8;TNCON），2） TN 和 MitoQ （n = 8;TNMitoQ），3） HS 对照 （n = 8;HSCON），或 4） HS 和 MitoQ （n = 8;HSMitoQ）。将猪移至单独的猪栏中并使其适应 6 天。该研究包括 2 个实验期 （P）。在 P1 （2 d） 期间，所有猪保持在 TN 条件 （20.6 ± 1.5 °C） 并随意饲喂。在 P2 （24 h） 期间，随意饲喂猪并暴露于 TN 或恒定 HS （37.3 ± 1.3 °C）。米托喹醇 （40 mg/d） 在 P1 和 P2 期间每天口服两次 （0700 和 1800 小时）。正如预期的那样，暴露于 HS 的猪直肠温度、皮肤温度和呼吸频率分别升高（+1.5 °C、+8.7 °C 和 +86 bpm;P < 0.01），与 TN 同类产品相比。与 TN 相比，HS 猪的采食量降低 （67%;P < 0.01） 和显著的 BW 损失 （+1.5 vs. -1.9 kg，分别为;P < 0.01）。相对于 TN 猪，HS 的总 GIT 重量降低 （P < 0.01），这受到管腔内容物降低的影响 （分别为 2.43 和 3.26 kg;P < 0.01） 和空 GIT 质量减少 （分别为 3.21 vs. 3.48 kg;P = 0.03）。TN 和 HS 猪的胃内容物保持相似 （P > 0.54），但相对于 CON 猪 （分别为 0.90 vs. 0.63 kg;P = 0.08）。 与 TN 对照相比，HS 的胃内容物占前 24 小时采食量的百分比增加（93% 对 31%;P < 0.01）。相比之下，与 TN 猪相比，HS 的小肠内容物和大肠内容物降低（分别为 23% 和 49%;P < 0.01）。相对于 TN 猪，HS 的肝脏重量下降（分别为 1.15 和 1.22 kg;P = 0.02），并且与 CON 猪相比，MitoQ 降低 （1.13 vs. 1.24 kg;P < 0.01）。相对于 TN 猪 （16%;P = 0.07）。总之，急性 HS 增加了所有体温指数，对动物生产性能产生了负面影响，并差异地改变了 GIT 运动，胃排空减少和肠道转运增加证明了这一点。然而，MitoQ 补充剂似乎并没有改善这些影响。