Recent respiratory outbreaks have garnered substantial attention, yet most respiratory monitoring remains confined to physical signals. Exhaled breath condensate (EBC) harbors rich molecular information that could unveil diverse insights into an individual’s health. Unfortunately, challenges related to sample collection and the lack of on-site analytical tools impede the widespread adoption of EBC analysis. Here, we introduce EBCare, a mask-based device for real-time in situ monitoring of EBC biomarkers. Using a tandem cooling strategy, automated microfluidics, highly selective electrochemical biosensors, and a wireless reading circuit, EBCare enables continuous multimodal monitoring of EBC analytes across real-life indoor and outdoor activities. We validated EBCare’s usability in assessing metabolic conditions and respiratory airway inflammation in healthy participants, patients with chronic obstructive pulmonary disease or asthma, and patients after COVID-19 infection.

中文翻译：

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用于收集和分析呼出气体冷凝物的智能面罩


最近的呼吸道疾病爆发引起了广泛关注，但大多数呼吸监测仍然仅限于物理信号。呼出气冷凝物 (EBC) 蕴藏着丰富的分子信息，可以揭示对个人健康的不同见解。不幸的是，与样本收集相关的挑战和缺乏现场分析工具阻碍了 EBC 分析的广泛采用。在这里，我们介绍 EBCare，一种基于面罩的设备，用于实时原位监测 EBC 生物标志物。 EBCare 采用串联冷却策略、自动化微流体、高选择性电化学生物传感器和无线读取电路，能够在现实生活中的室内和室外活动中对 EBC 分析物进行连续多模式监测。我们验证了 EBCare 在评估健康参与者、慢性阻塞性肺病或哮喘患者以及 COVID-19 感染后患者的代谢状况和呼吸道炎症方面的可用性。