Ultrabroadband Optical Diffraction Tomography,ACS Photonics

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Ultrabroadband Optical Diffraction Tomography
ACS Photonics ( IF 6.5 ) Pub Date : 2024-08-27 , DOI: 10.1021/acsphotonics.4c00797
Martin Hörmann ₁ , Franco V. A. Camargo ₂ , Niek F. van Hulst _{3,

4} , Giulio Cerullo _{1,

2} , Matz Liebel _{3,

5}

Affiliation

Optical diffraction tomography (ODT) is a powerful noninvasive 3D imaging technique, but its combination with broadband light sources is difficult. In this study, we introduce ultrabroadband ODT, covering over 150 nm of visible spectral bandwidth with a lateral spatial resolution of 150 nm. Our work addresses a critical experimental gap by enabling the measurement of broadband refractive index changes in 3D samples, crucial information that is difficult to assess with existing methodologies. We present broadband, spectrally resolved ODT images of HeLa cells, obtained via pulse-shaping-based Fourier transform spectroscopy. The spectral observations enabled by ultrabroadband ODT, combined with material-dependent refractive index responses, allow for precise three-dimensional identification of nanoparticles within cellular structures. Our work represents a crucial step toward time and spectrally resolved tomography of complex 3D structures with implications for life and materials science applications.

中文翻译：

超宽带光学衍射断层扫描

光学衍射断层扫描（ODT）是一种强大的非侵入性3D成像技术，但其与宽带光源的结合很困难。在这项研究中，我们引入了超宽带 ODT，覆盖超过 150 nm 的可见光谱带宽，横向空间分辨率为 150 nm。我们的工作通过测量 3D 样品中的宽带折射率变化来解决一个关键的实验差距，这是用现有方法难以评估的关键信息。我们展示了 HeLa 细胞的宽带光谱解析 ODT 图像，这些图像是通过基于脉冲整形的傅里叶变换光谱获得的。超宽带 ODT 实现的光谱观察与材料相关的折射率响应相结合，可以对细胞结构内的纳米颗粒进行精确的三维识别。我们的工作代表了复杂 3D 结构的时间和光谱分辨断层扫描的关键一步，对生命和材料科学应用具有重要意义。

更新日期：2024-08-27

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