Spatiotemporal Three-Dimensional Quantitative Visualization of Macrophage Phagocytosis of Adjuvants In Vivo Using Optical-Resolution Photoacoustic Microscopy,ACS Photonics

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Spatiotemporal Three-Dimensional Quantitative Visualization of Macrophage Phagocytosis of Adjuvants In Vivo Using Optical-Resolution Photoacoustic Microscopy
ACS Photonics ( IF 6.5 ) Pub Date : 2024-08-26 , DOI: 10.1021/acsphotonics.4c01106
Fengbing He _{1,

2} , Dong Liu ₂ , Yiqing Zhang ₂ , Fan Meng ₂ , Chaohao Liang ₂ , Wuyu Zhang ₃ , Zixi Liu ₂ , Jian Zhang _{1,

2}

Affiliation

As a crucial component of vaccines, adjuvants rely on the ability of macrophages to phagocytose for immune activation. It is extremely valuable to capture this complex process accurately. In this study, we constructed an optical-resolution photoacoustic microscopy (OR-PAM) system with lateral and axial resolutions of 630 nm and 2.52 μm, respectively, enabling high-resolution three-dimensional visualization and quantitative analysis of the adjuvant phagocytosis process of macrophages. OR-PAM results showed that compared with undifferentiated monocyte macrophages (M0), classically activated macrophages (M1) had profound phagocytic ability to label CpG oligodeoxynucleotides with gold nanoparticles (AuNPs) (CpG–Au) and aluminum adjuvant labeled with lumogallion (Alum–Ga), consistent with the phagocytic observations of AuNPs. In addition, the TNF-α, IL-6, and IL-1β released by M1 were significantly higher than those released by M0 within 2 h after CpG–Au and Alum–Ga stimulation. These results indicated that the size of the adjuvant can affect the phagocytosis efficiency of the macrophages. The findings provide a better understanding of the adjuvant-induced macrophage response and help to develop more efficient and specific vaccines.

中文翻译：

使用光学分辨率光声显微镜对体内佐剂的巨噬细胞吞噬作用进行时空三维定量可视化

作为疫苗的重要组成部分，佐剂依赖巨噬细胞的吞噬能力来激活免疫。准确捕捉这一复杂的过程非常有价值。在本研究中，我们构建了横向和轴向分辨率分别为630 nm和2.52 μm的光学分辨率光声显微镜（OR-PAM）系统，能够对巨噬细胞的辅助吞噬过程进行高分辨率三维可视化和定量分析。 OR-PAM结果表明，与未分化的单核巨噬细胞（M0）相比，经典活化的巨噬细胞（M1）具有很强的吞噬能力，可以用金纳米粒子（AuNPs）（CpG-Au）和用lumogallion标记的铝佐剂（Alum-Ga）标记CpG寡脱氧核苷酸。），与 AuNPs 的吞噬细胞观察结果一致。此外，在CpG-Au和Alum-Ga刺激后2小时内，M1释放的TNF-α、IL-6和IL-1β显着高于M0释放的TNF-α、IL-6和IL-1β。这些结果表明佐剂的大小可以影响巨噬细胞的吞噬效率。这些发现提供了对佐剂诱导的巨噬细胞反应的更好理解，并有助于开发更有效和特异性的疫苗。

更新日期：2024-08-26

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