As investigations of low-biomass microbial communities have become more common, so too has the recognition of major challenges affecting these analyses. These challenges have been shown to compromise biological conclusions and have contributed to several controversies. Here, we review some of the most common and influential challenges in low-biomass microbiome research. We highlight key approaches to alleviate these potential pitfalls, combining experimental planning strategies and data analysis methods.

中文翻译：

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规划和分析低生物量微生物组研究：数据分析视角


随着对低生物量微生物群落的研究变得越来越普遍，人们也认识到影响这些分析的主要挑战。这些挑战已被证明会损害生物学结论，并引发了一些争议。在这里，我们回顾了低生物量微生物组研究中一些最常见和最有影响力的挑战。我们重点介绍了缓解这些潜在陷阱的关键方法，并结合了实验规划策略和数据分析方法。