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New findings on the genetic basis of feathered legs in chickens: association of CUBN gene mutations with feathered-leg phenotype
Journal of Animal Science ( IF 2.7 ) Pub Date : 2024-08-25 , DOI: 10.1093/jas/skae252
Lan Huang 1, 2 , Qixin Guo 2 , Jialuo Sun 2 , Zhixiu Wang 2 , Yong Jiang 2 , Guobin Chang 2 , Hao Bai 1, 2
Affiliation  

Chickens are the most thoroughly domesticated vertebrate species, and after long-continued natural and artificial selection, they now show rich phenotypic diversity. In particular, feathered legs present in domestic chickens are a characteristic that is carefully selected by advanced breeders. Previous studies have identified the key mutations responsible for feathered legs on chromosomes 13 and 15; however, not all chickens can be easily distinguished based on these two markers. In this study, whole-genome resequencing of 29 Bamaxiaogu chickens (BXCs) yielded 12,201,978 valid single-nucleotide polymorphisms (SNPs) and 2,792,426 valid insertions and deletions (InDels). Population structure analysis based on SNPs revealed that the test samples came from the same natural population. Based on these findings, we used an SNP- and InDel-based genome-wide association study (GWAS) to investigate the genetic basis of feathered legs in chickens. GWAS results revealed that 2 SNPs located in the introns of cubilin (CUBN; SNP1, chr2:19885382T>A) and recombinant Ras suppressor protein 1 (RSU1) genes (SNP2, chr2:20002551G>A), as well as an InDel (InDel1, chr2:19884383TG>T) on CUBN, were all significantly associated with the presence of feathered legs. Diagnostic testing demonstrated that SNP1 effectively differentiated between chickens with feathered legs and those with clean legs (leg without feathers) within the BXC population and may thus be considered an effective marker of feathered legs in BXC. In contrast, other loci did not show the same discriminatory power. This study not only presents a new variant of feathered legs but also provides valuable novel insights into the underlying mechanisms of variation in the feathered-legs trait among chickens.

中文翻译:


鸡羽腿遗传基础的新发现:CUBN 基因突变与羽腿表型的关系



鸡是驯化最彻底的脊椎动物物种,经过长期持续的自然和人工选择,它们现在显示出丰富的表型多样性。特别是,家鸡中存在的羽毛腿是高级饲养员精心挑选的特征。以前的研究已经确定了导致 13 号和 15 号染色体上羽毛腿的关键突变;然而,并非所有鸡都可以根据这两个标记轻松区分。在本研究中,对 29 只八马小谷鸡 (BXCs) 进行全基因组重测序,获得 12,201,978 个有效的单核苷酸多态性 (SNP) 和 2,792,426 个有效的插入和缺失 (InDels)。基于 SNPs 的种群结构分析表明,测试样本来自同一自然种群。基于这些发现,我们使用基于 SNP 和 InDel 的全基因组关联研究 (GWAS) 来研究鸡羽毛腿的遗传基础。GWAS结果显示,2个SNP位点位于cubilin的内含子中(CUBN;SNP1, chr2:19885382T>A) 和重组 Ras 抑制蛋白 1 (RSU1) 基因 (SNP2, chr2:20002551G>A) 以及 CUBN 上的 InDel (InDel1, chr2:19884383TG>T) 都与羽毛腿的存在显著相关。诊断测试表明,SNP1 在 BXC 种群中有效区分了有羽毛腿的鸡和有羽毛腿的鸡(没有羽毛的腿),因此可能被认为是 BXC 中羽毛腿的有效标志物。相比之下,其他基因座没有表现出相同的区分力。这项研究不仅提出了一种新的羽毛腿变体,而且还为鸡羽毛腿性状变异的潜在机制提供了有价值的新见解。
更新日期:2024-08-25
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