This study examines the effects of Roundup Transorb® (RDT) exposure on reproductive functions and ovarian miRNA expression in . Exposure to RDT (at 0.065 or 5 mg. L for 96 h) significantly disrupts fertility, evidenced by changes in fertilization rates and egg diameter. Profiling of ovarian miRNAs identified a total 205 miRNAs in . Among these, three miRNAs were upregulated (miR-10b-5p, miR-132–3p, miR-100–5p), while ten miRNAs were downregulated (miR-499–5p, miR-375, miR-205–5p, miR-206–3p, miR-203a-3p, miR-133b-3p, miR-203b-5p, miR-184, miR-133a-3p, miR-2188–5p) compared to non-exposed fish. This study reveals that differentially expressed miRNAs are linked to molecular pathways such as steroid hormone biosynthesis, lipid and carbohydrate metabolism, bioenergetics, and antioxidant defense. It also analyzes molecular interactions between miRNAs and target genes during RDT exposure in annual killifish, providing insights into biomarkers in ecotoxicology. Moreover, it provides scope for developing environmental health assessment models based on epigenomic endpoints, supporting the protection of biodiversity and ecosystem services through the quantification of stress responses in living organisms exposed to pesticides.

中文翻译：

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评估接触农达 Transorb® 的澳洲甜椒的生殖效应和表观遗传反应：来自 miRNA 分析和分子相互作用分析的见解


本研究探讨了农达 Transorb® (RDT) 暴露对生殖功能和卵巢 miRNA 表达的影响。暴露于 RDT（0.065 或 5 mg.L，持续 96 小时）会显着破坏生育能力，受精率和卵子直径的变化证明了这一点。卵巢 miRNA 分析共识别出 205 个 miRNA。其中，3个miRNA上调（miR-10b-5p，miR-132–3p，miR-100–5p），而10个miRNA下调（miR-499–5p，miR-375，miR-205–5p，miR -206–3p、miR-203a-3p、miR-133b-3p、miR-203b-5p、miR-184、miR-133a-3p、miR-2188–5p）与未暴露的鱼相比。这项研究揭示了差异表达的 miRNA 与类固醇激素生物合成、脂质和碳水化合物代谢、生物能量学和抗氧化防御等分子途径有关。它还分析了一年生鳉鱼 RDT 暴露期间 miRNA 和靶基因之间的分子相互作用，为生态毒理学中的生物标志物提供了见解。此外，它还为开发基于表观基因组终点的环境健康评估模型提供了空间，通过量化接触农药的生物体的应激反应来支持保护生物多样性和生态系统服务。