A lateral strip assay for ultrasensitive detection of glyphosate in soybeans and corn,Analyst

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A lateral strip assay for ultrasensitive detection of glyphosate in soybeans and corn
Analyst ( IF 3.6 ) Pub Date : 2024-08-26 , DOI: 10.1039/d4an00958d
Xuyang Ma _{1,

2} , Liqiang Liu _{1,

2} , Shanshan Song _{1,

2} , Hua Kuang _{1,

2} , Chuanlai Xu _{1,

2} , Xinxin Xu _{1,

2}

Affiliation

Glyphosate (GLY) is widely applied in agriculture and horticulture as a herbicide. The development of genetically modified plants has caused abuse of GLY, with excessive residues potentially causing harm to human health. Consequently, a novel method needs to be built to detect GLY in soybeans and corn. Computer simulation was used to design an excellent hapten which was used to produce an anti-GLY monoclonal antibody (mAb) with outstanding sensitivity and affinity, and its 50%-inhibitory concentration (IC₅₀) was 128.59 ng mL⁻¹. Afterwards, an immunochromatographic assay strip was developed based on the mAb. In soybeans and corn, the visual detection limits were 1 mg kg⁻¹ and 0.2 mg kg⁻¹, while the cut-off values were 50 mg kg⁻¹ and 5 mg kg⁻¹, respectively. The reliability of the strips was proved by the existing methods. Thus, a rapid method to detect GLY residues on-site in soybeans and corn was established.

中文翻译：

用于大豆和玉米中草甘膦超灵敏检测的侧向条带检测

草甘膦（GLY）作为除草剂广泛用于农业和园艺。转基因植物的发展导致了 GLY 的滥用，过量的残留物可能对人类健康造成危害。因此，需要建立一种新的方法来检测大豆和玉米中的 GLY。使用计算机模拟设计了一种出色的半抗原，用于生产具有出色灵敏度和亲和力的抗 GLY 单克隆抗体（mAb），其 50% 抑制浓度（IC₅₀）为 128.59 ng ^mL-1。之后，基于 mAb 开发了免疫层析检测条。在大豆和玉米中，肉眼检测限为 1 mg ^kg-1 和 0.2 mg ^kg-1，而临界值分别为 50 mg ^kg-1 和 5 mg ^kg-1。现有方法证明了条带的可靠性。因此，建立了一种在大豆和玉米中现场检测 GLY 残留物的快速方法。

更新日期：2024-08-26

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