Membrane remodeling drives a broad spectrum of cellular functions, and it is regulated through mechanical forces exerted on the membrane by cytoplasmic complexes. Here, we investigate how actin filaments dynamically tune their structure to control the active transfer of membranes between cellular compartments with distinct compositions and biophysical properties. Using intravital subcellular microscopy in live rodents we show that a lattice composed of linear filaments stabilizes the granule membrane after fusion with the plasma membrane and a network of branched filaments linked to the membranes by Ezrin, a regulator of membrane tension, initiates and drives to completion the integration step. Our results highlight how the actin cytoskeleton tunes its structure to adapt to dynamic changes in the biophysical properties of membranes.

中文翻译：

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基于肌动蛋白的产力模块的协调可稳定和重塑体内膜。


膜重塑驱动广泛的细胞功能，并通过细胞质复合物施加在膜上的机械力进行调节。在这里，我们研究肌动蛋白丝如何动态调整其结构以控制具有不同组成和生物物理特性的细胞区室之间膜的主动转移。使用活体啮齿动物的活体亚细胞显微镜，我们发现由线性细丝组成的晶格在与质膜融合后稳定颗粒膜，并且通过膜张力调节剂埃兹林（Ezrin）与膜连接的分支细丝网络启动并驱动完成整合步骤。我们的结果强调了肌动蛋白细胞骨架如何调整其结构以适应膜生物物理特性的动态变化。