Genome editing technologies are rapidly evolving, from the early zinc-finger nucleases, transcription activator-like effector nucleases (TALENs), and CRISPR-Cas9 (Figure 1, initial genome editing technologies), which generate double-strand breaks (DSBs), to base editing, which makes precise nucleobase conversion without inducing DSBs, and prime editing, which can carry out all types of edits without DSBs or donor DNA templates. The emergence of these revolutionary technologies offers us unprecedented opportunities for biomedical research and therapy development.

中文翻译：

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快速发展的基因组和表观基因组编辑技术


基因组编辑技术正在迅速发展，从产生双链断裂 (DSB) 的早期锌指核酸酶、类转录激活子效应核酸酶 (TALEN) 和 CRISPR-Cas9（图 1，最初的基因组编辑技术），到碱基编辑可在不诱导 DSB 的情况下进行精确的核碱基转换，而 Prime 编辑可在无需 DSB 或供体 DNA 模板的情况下进行所有类型的编辑。这些革命性技术的出现为我们的生物医学研究和治疗开发提供了前所未有的机遇。