Fibronectin (FN) is a ubiquitous extracellular matrix glycoprotein essential for the development of various tissues. Mutations in FN cause a unique form of spondylometaphyseal dysplasia, emphasizing its importance in cartilage and bone development. However, the relevance and functional role of FN during skeletal development has remained elusive. To address these aspects, we have generated conditional knockout mouse models targeting the cellular FN isoform in cartilage (cFNKO), the plasma FN isoform in hepatocytes (pFNKO), and both isoforms together in a double knockout (FNdKO). We used these mice to determine the relevance of the two principal FN isoforms in skeletal development from postnatal day one to the adult stage at two months.

中文翻译：

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纤连蛋白亚型促进产后骨骼发育


纤连蛋白 (FN) 是一种普遍存在的细胞外基质糖蛋白，对于各种组织的发育至关重要。 FN 突变会导致一种独特形式的脊柱干骺端发育不良，强调其在软骨和骨骼发育中的重要性。然而，FN 在骨骼发育过程中的相关性和功能作用仍然难以捉摸。为了解决这些问题，我们生成了针对软骨中的细胞 FN 同工型 (cFNKO)、肝细胞中的血浆 FN 同工型 (pFNKO) 以及双敲除中的两种同工型 (FNdKO) 的条件敲除小鼠模型。我们使用这些小鼠来确定两种主要 FN 亚型在从出生后第一天到成年阶段两个月的骨骼发育中的相关性。