In this study, pressurized hot water extraction (PHWE) was evaluated for the recovery of anti-diabetic borapetoside C (BPC) from stems. The maximum BPC extraction efficiency obtained at 100 ˚C, 2.5 MPa and 5.0 mL/min was considerably higher than that obtained by the conventional methods. Under optimized conditions, one-site kinetic desorption model could most accurately describe the PHWE behavior, suggesting an intra-particle diffusion-controlled mechanism. The undesirable compounds in the extract were further removed by micelle-mediated separation (MMS), in which Tween 80 was added, followed by NaCl addition and slight temperature increase to induce phase separation. At the most suitable MMS condition, with 0.028 mM Tween 80, 0.4 M NaCl, at 85 ˚C, the majority (87 %) of BPC could be recovered in the aqueous phase after 40 min. After MMS, the resulting extract exhibited low cytotoxicity against L6 and HepG2 cells while maintaining significant α-glucosidase and α-amylase inhibition activities.

中文翻译：

---

通过加压热水提取和胶束介导的分离的可持续方法，增强卷草提取物的抗糖尿病活性并降低细胞毒性


在本研究中，评估了加压热水提取 (PHWE) 从茎中回收抗糖尿病药物博拉皮苷 C (BPC) 的情况。在 100 ℃、2.5 MPa 和 5.0 mL/min 下获得的最大 BPC 提取效率明显高于传统方法获得的提取效率。在优化条件下，单点动力学解吸模型可以最准确地描述 PHWE 行为，表明颗粒内扩散控制机制。通过胶束介导的分离 (MMS) 进一步去除提取物中不需要的化合物，其中添加 Tween 80，然后添加 NaCl 并稍微升高温度以诱导相分离。在最合适的 MMS 条件下，使用 0.028 mM Tween 80、0.4 M NaCl，在 85 ℃ 下，40 分钟后，大部分 (87%) BPC 可在水相中回收。 MMS 后，所得提取物对 L6 和 HepG2 细胞表现出低细胞毒性，同时保持显着的 α-葡萄糖苷酶和 α-淀粉酶抑制活性。