Malignant melanoma is the most lethal form of skin cancer. As a promising anti-cancer agent, plasma-activated water (PAW) rich in reactive oxygen and nitrogen species (RONS) has shown significant potential for melanoma treatment. However, rapid decay of RONS and inefficient delivery of PAW in conventional injection methods limit its practical applications. To address this issue, here we report a new approach for the production of plasma-activated cryo-microneedles (PA-CMNs) patches using custom-designed plasma devices and processes. Our innovation is to incorporate PAW into the PA-CMNs that are fabricated using a fast cryogenic micro-molding method. It is demonstrated that PA-CMNs can be easily inserted into skin to release RONS and slow the decay of RONS thereby prolonging their bioactivity and effectiveness. The new insights into the effective melanoma treatment suggest that the rich mixture of RONS within PA-CMNs prepared by custom-developed hybrid plasma-assisted configuration induces both ferroptosis and apoptosis to selectively kill tumor cells. A significant inhibition of subcutaneous A375 melanoma growth was observed in PA-CMNs-treated tumor-bearing nude mice without any signs of systemic toxicity. The new approach based on PA-CMNs may potentially open new avenues for a broader range of disease treatments.

中文翻译：

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将液体中的等离子体产生的 RONS 转移到冷冻微针贴片中，用于黑色素瘤的皮肤治疗。


恶性黑色素瘤是最致命的皮肤癌。作为一种有前景的抗癌剂，富含活性氧和活性氮（RONS）的等离子体活化水（PAW）在治疗黑色素瘤方面显示出巨大的潜力。然而，传统注射方法中 RONS 的快速衰减和 PAW 的低效传递限制了其实际应用。为了解决这个问题，我们在这里报告了一种使用定制设计的等离子体设备和工艺生产等离子体激活冷冻微针（PA-CMN）贴片的新方法。我们的创新是将 PAW 融入使用快速低温微成型方法制造的 PA-CMN 中。研究表明，PA-CMNs 可以很容易地插入皮肤中释放 RONS 并减缓 RONS 的衰减，从而延长其生物活性和有效性。对有效黑色素瘤治疗的新见解表明，通过定制开发的混合等离子体辅助配置制备的 PA-CMN 内丰富的 RONS 混合物可诱导铁死亡和细胞凋亡，从而选择性杀死肿瘤细胞。在 PA-CMNs 治疗的荷瘤裸鼠中观察到皮下 A375 黑色素瘤生长受到显着抑制，且没有任何全身毒性迹象。基于 PA-CMN 的新方法可能为更广泛的疾病治疗开辟新途径。