The present study elucidates the effectiveness of a molecularly imprinted polyacrylonitrile-imbued graphite-base electrode (MAN@G) for the selective detection of folic acid (FA) in food samples. The prime objective of the recognition and quantification of vitamin compounds like FA is the overall quality assessment of vegetables and fruits. The cost-effective, reproducible, and durable MAN@G electrode has been fabricated using acrylonitrile (AN) as the monomer and FA as the template over graphite-base. The characterization of the synthesized MAN@G electrode material has been accomplished by utilizing UV–visible (UV–vis) spectroscopy and scanning electron microscopy (SEM). A tri-electrode system based on differential pulse voltammetry (DPV) and cyclic voltammetry (CV) techniques was employed to explore the analytical performance of the synthesized electrode. Rigorous analyses divulged that a widespread linearity window could be exhibited by the electrode under an optimized experimental environment, ranging from 20 μM to 400 μM concentrations with an acceptable lower limit of detection (LOD) and limit of quantification (LOQ) of 18 nM, and 60 nM respectively. Additionally, this electrode exhibits high reproducibility, good stability, and high repeatability, with RSD values of 1.72 %, 1.32 %, and 1.19 %, respectively. The detection efficacy of the proposed electrode has been further examined in food extracts, namely orange, spinach, papaya, soybean, and cooked rice, which endorsed high accuracy compared to the high-performance liquid chromatography (HPLC) method. Moreover, the statistical results obtained from the -test analysis were also satisfactory for the FA concentrations present in those five samples.

中文翻译：

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使用分子印迹聚丙烯腈浸渍石墨电极电化学检测食品提取物中的叶酸


本研究阐明了分子印迹聚丙烯腈石墨基电极 (MAN@G) 在选择性检测食品样品中叶酸 (FA) 方面的有效性。 FA 等维生素化合物的识别和定量的主要目标是对蔬菜和水果的整体质量评估。这种经济高效、可重复且耐用的 MAN@G 电极是使用丙烯腈 (AN) 作为单体和 FA 作为石墨基模板制成的。合成的 MAN@G 电极材料的表征是通过利用紫外可见 (UV-vis) 光谱和扫描电子显微镜 (SEM) 完成的。采用基于微分脉冲伏安法（DPV）和循环伏安法（CV）技术的三电极系统来探索合成电极的分析性能。严格的分析表明，在优化的实验环境下，电极可以表现出广泛的线性窗口，浓度范围为 20 μM 至 400 μM，可接受的检测下限 (LOD) 和定量限 (LOQ) 为 18 nM，并且分别为60nM。此外，该电极具有高重现性、良好稳定性和高重复性，RSD 值分别为 1.72 %、1.32 % 和 1.19 %。所提出的电极的检测效果在橙子、菠菜、木瓜、大豆和米饭等食品提取物中得到了进一步检验，与高效液相色谱（HPLC）方法相比，其准确性较高。此外，从测试分析中获得的统计结果对于这五个样品中存在的 FA 浓度也令人满意。