β-nicotinamide mononucleotide stands out as an essential breakthrough in “anti-aging” and consistently leads the list of top-selling nutritional supplements in terms of quantity. As the metabolites of β-nicotinamide mononucleotide, the detection of nicotinamide and N-methylnicotinamide is of great significance for evaluating the nutritional effect of dietary supplements of β-nicotinamide mononucleotide. However, due to the extremely low concentration of nicotinamide and N-methylnicotinamide in vivo and the serious matrix interference in biological samples, there is an increasing demand for materials and methods of pre-treatment. In this study, FeO@hydroxypropyl methyl cellulose@dodecylbenzenesulfonic acid magnetic fluid was synthesized for the first time, and it was used as pretreatment material to detect nicotinamide and N- methylnicotinamide in urine samples by high performance liquid chromatography. Compared with other adsorption materials, FeO@hydroxypropyl methyl cellulose@dodecylbenzenesulfonic acid nanoparticles are a kind of uniform magnetic fluid. Furthermore, they have more types and quantities of interaction sites (electrostatic interactions, hydrophobic interactions, hydrogen bonding interactions, and π-π interactions), so they own greater adsorption capacity. When the pH of the solution is 4, they can be adsorbed quickly within 10 s. The method successfully detected trace nicotinamide and N-methylnicotinamide in urine samples in the linear range of 0.1–80 μg mL, the low detection limits were 0.30 ng mL and 1.5 ng mL respectively, and the quantification limits were 1.0 ng mL and 5.0 ng mL, respectively. At the same time, the standard urine samples of nicotinamide and N-methylnicotinamide showed satisfactory recovery rate 94.50–109.1 %. The results indicated that the established method can accurately and quantitatively determine trace nicotinamide and N-methylnicotinamide in urine samples. Consequently, low concentration of β-nicotinamide mononucleotide metabolites can be detected simultaneously, and the interference can be eliminated during the detection process, which provides an efficient and convenient pretreatment method and a rapid and sensitive detection method for the analysis of β-nicotinamide mononucleotide metabolites. What's more, it has a wide application prospect in the analysis of other similar metabolites in biological samples.

中文翻译：

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多重相互作用磁流体的制备用于快速、灵敏地同时提取生物样品中的β-烟酰胺单核苷酸代谢物


β-烟酰胺单核苷酸在“抗衰老”方面取得了重大突破，在销量上一直稳居营养补充剂销量榜首。作为β-烟酰胺单核苷酸的代谢产物，烟酰胺和N-甲基烟酰胺的检测对于评价β-烟酰胺单核苷酸膳食补充剂的营养效果具有重要意义。然而，由于烟酰胺和N-甲基烟酰胺在体内的浓度极低，且生物样品中基质干扰严重，对前处理的材料和方法的需求越来越大。本研究首次合成了FeO@羟丙基甲基纤维素@十二烷基苯磺酸磁流体，并将其作为前处理材料，采用高效液相色谱法检测尿液样本中的烟酰胺和N-甲基烟酰胺。与其他吸附材料相比，Fe3O@羟丙基甲基纤维素@十二烷基苯磺酸纳米粒子是一种均匀的磁流体。此外，它们具有更多类型和数量的相互作用位点（静电相互作用、疏水相互作用、氢键相互作用、π-π相互作用），因此具有更大的吸附能力。当溶液的pH为4时，它们可以在10秒内被快速吸附。该方法成功检测出尿样中痕量烟酰胺和N-甲基烟酰胺，线性范围为0.1~80 μg·mL，检出低限分别为0.30 ng·mL和1.5 ng·mL，定量限分别为1.0 ng·mL和5.0 ng·mL ， 分别。同时，烟酰胺和N-甲基烟酰胺标准尿样的回收率令人满意，达到94.50%~109.1%。 结果表明，所建立的方法能够准确定量测定尿样中痕量烟酰胺和N-甲基烟酰胺。因此，可以同时检测低浓度的β-烟酰胺单核苷酸代谢物，并且消除检测过程中的干扰，为β-烟酰胺单核苷酸代谢物的分析提供了高效、便捷的前处理方法和快速、灵敏的检测方法。 。更重要的是，它在生物样品中其他类似代谢物的分析中具有广泛的应用前景。