Recent studies have shown that age-related aging evolution is accompanied by imbalances in intestinal homeostasis. Marine red yeast (MRY) is a functional probiotic that has been shown to have antioxidant, immune and other properties. Therefore, we chose 900 healthy Hy-Line Brown hens at 433 d old as the research subjects and evaluated the correlation between intestinal health, laying performance, and egg quality in aged hens through the supplementation of MRY. These laying hens were assigned into 5 groups and received diet supplementation with 0%, 0.5%, 1.0%, 1.5%, and 2% MRY for 12 weeks. The results showed that MRY supplementation increased egg production rate, average egg weight, and egg quality, and decreased feed conversion ratio and daily feed intake (P < 0.05). The MRY supplement improved antioxidant indicators such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), stimulated villus height, and increased the villus height to crypt depth ratio (V/C ratio) in the intestine (P < 0.05). It also regulated the expression of intestinal inflammatory factors (transforming growth factor-β [TGF-β], interleukin [IL]-1β, IL-8, tumor necrosis factor-α [TNF-α]) while increasing serum immunoglobulin G (IgG) levels (P < 0.05). Furthermore, MRY supplementation upregulated the mRNA expression of tight junction proteins (occludin and zonula occludens-1 [ZO-1]), anti-apoptotic gene (Bcl-2), and autophagy-related proteins (beclin-1 and light chain 3I [LC3I]) in the intestine (P < 0.05). The MRY supplement also led to an increase in the concentration of short-chain fatty acids in the cecum, and the relative abundance of the phylum Bacteroidetes, and genera Bacteroides and Rikenellaceae_RC9_gut_group. The LEfSe analysis revealed an enrichment of Sutterella and Akkermansia muciniphila. In conclusion, the results of this experiment indicated that the additional supplementation of MRY can improve the production performance of laying hens and may contribute to the restoration and balance of intestinal homeostasis, which supports the application potential of MRY as a green and efficient feed additive for improving the laying performance in chickens.

中文翻译：

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补充海洋红曲通过调节老年鸡的小肠稳态来改善产蛋性能


最近的研究表明，与年龄相关的衰老进化伴随着肠道稳态的失衡。海洋红曲 （MRY） 是一种功能性益生菌，已被证明具有抗氧化、免疫和其他特性。因此，我们选择了 900 只 433 d 龄的健康 Hy-Line Brown 母鸡作为研究对象，并通过补充 MRY 评估了老年母鸡肠道健康、产蛋性能和蛋品质之间的相关性。将这些蛋鸡分为 5 组，并接受 0% 、 0.5% 、 1.0% 、 1.5% 和 2% MRY 的日粮补充剂，持续 12 周。结果表明，添加 MRY 提高了产蛋率、平均蛋重和蛋品质，降低了饲料转化率和日采食量 （P < 0.05）。MRY 补充剂改善了超氧化物歧化酶 （SOD） 、过氧化氢酶 （CAT）、谷胱甘肽过氧化物酶 （GSH-Px） 等抗氧化指标，刺激了绒毛高度，并增加了肠道绒毛高度与隐窝深度比 （V/C 比值） （P < 0.05）。它还调节肠道炎症因子 （转化生长因子-β [TGF-β]、白细胞介素 [IL]-1β、IL-8、肿瘤坏死因子-α [TNF-α]）的表达，同时提高血清免疫球蛋白 G （IgG） 水平 （P < 0.05）。此外，MRY 补充剂上调肠道中紧密连接蛋白 （occludin 和 zonula occludens-1 [ZO-1]）、抗凋亡基因 （Bcl-2） 和自噬相关蛋白 （beclin-1 和轻链 3I [LC3I]） 的 mRNA 表达 （P < 0.05）。MRY 补充剂还导致盲肠中短链脂肪酸浓度的增加，以及拟杆菌门、拟杆菌属和拟杆菌属Rikenellaceae_RC9_gut_group的相对丰度增加。 LEfSe 分析显示 Sutterella 和 Akkermansia muciniphila 的富集。综上所述，本实验结果表明，额外添加 MRY 可以提高蛋鸡的生产性能，并可能有助于肠道稳态的恢复和平衡，这支持 MRY 作为绿色高效饲料添加剂在提高鸡产蛋性能方面的应用潜力。