Nanoplastics (NPs) affect fertility. We evaluated the effects of NPs treatment on luteal and endothelial cells. We examined crucial markers of growth and redox status. NPs treatment did not induce changes in ATP levels in luteal cells, while it increased (p< 0.05) their proliferation. In endothelial cells, no change in proliferation was detected, while an increase (p<0.05) in ATP levels was observed. The increase of reactive oxygen species, superoxide anion (p<0.05) and nitric oxide (p<0.001) was detected in both cell types, which also showed changes in superoxide dismutase enzyme activity as well as an increase of non-enzymatic antioxidant power (p<0.05). A decrease (p<0.05) in progesterone production as well as an increase of vascular endothelial growth factor A levels were detected (p<0.05). In addition, a dose-dependent accumulation of NPs in endothelial cells was shown, that likely occurred through adhesion and internalization. Results underline potential risk of NPs for corpus luteum functionality.

中文翻译：

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猪黄体作为研究纳米塑料影响的模型


纳米塑料 (NP) 影响生育能力。我们评估了 NP 治疗对黄体和内皮细胞的影响。我们检查了生长和氧化还原状态的关键标志物。 NP 处理不会引起黄体细胞中 ATP 水平的变化，但会增加 (p< 0.05) 其增殖。在内皮细胞中，未检测到增殖变化，但观察到 ATP 水平增加 (p<0.05)。在两种细胞类型中均检测到活性氧、超氧阴离子 (p<0.05) 和一氧化氮 (p<0.001) 的增加，这也表明超氧化物歧化酶活性的变化以及非酶促抗氧化能力（p<0.05）。检测到黄体酮产生减少 (p<0.05) 以及血管内皮生长因子 A 水平增加 (p<0.05)。此外，纳米颗粒在内皮细胞中的积累呈剂量依赖性，这可能是通过粘附和内化发生的。结果强调了纳米粒子对黄体功能的潜在风险。