An ABC transporter-mediated transport and metabolism of the pesticide bentazone in rice (Oryza sativa L.),Journal of Advanced Research

当前位置： X-MOL 学术 › J. Adv. Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

An ABC transporter-mediated transport and metabolism of the pesticide bentazone in rice (Oryza sativa L.)
Journal of Advanced Research ( IF 11.4 ) Pub Date : 2024-08-20 , DOI: 10.1016/j.jare.2024.08.020
Yuxin Qiao ₁ , Yujue Wang ₂ , Yucheng Gu ₃ , Nan Zhang ₄ , Hong Yang ₄ , Jintong Liu ₄

Affiliation

Introduction

Bentazon (BNTZ) is a selective contact herbicide widely used to control field weeds for crop production. Excessive use of BNTZ leads to its accumulation in soils and crops, becoming an environmental contaminant. Therefore, investigation of the mechanisms for BNTZ detoxification and degradation in crops is fundamentally important to reduce crop contamination and ensure food safety.

Objectives

This study aims to elucidate the mechanism of detoxification and degradation pathways of the BNTZ complex in rice by creating transgenic lines expressing a rice ATP-binding cassette (OsABC) transporter gene through genetic engineering techniques combined with chemical analytical techniques and metabolomics approaches.

Methods

We established the rice transgenic lines overexpressing (OE) a rice OsABC transporter and its knockout lines by CRISPR-Cas9 to characterize the gene function and measured the accumulation of BNTZ residues in rice. The metabolites of BNTZ were characterized by LC/Q-TOF-HRMS/MS (Liquid chromatography/time of flight-high resolution mass spectrometry).

Results

Overexpression of OsABC significantly conferred rice resistance to BNTZ toxicity by increasing plant elongation, dry weight, and chlorophyll content, and significantly reducing cell membrane damage and BNTZ accumulation in rice tissues. Six different metabolites and ten conjugates were well defined in chemical structures. The reduced BNTZ levels and degradation products in the grains of the OE lines supported the robust activity of the OsABC gene function.Using UPLC-Q-TOF/MS, we further identified accumulated basic metabolites of various carbohydrates, amino acids, hormones, and flavonoids, and found that these metabolites involved in BNTZ degradation were increased more in OE lines than in wild-type (WT) rice.

Conclusions

Our work demonstrates that the OsABC transporter plays a critical role in regulating the mobility and degradative metabolism of BNTZ in rice, thus revealing a regulatory mechanism underlying rice resistance to BNTZ toxicity and adaptation to the environmental stress.

中文翻译：

ABC 转运蛋白介导的水稻中农药苯甲酮（Oryza sativa L.）的运输和代谢

介绍

Bentazon （BNTZ）是一种选择性接触除草剂，广泛用于控制农作物生产中的田间杂草。过量使用 BNTZ 会导致其在土壤和农作物中积累，成为环境污染物。因此，研究 BNTZ 在农作物中的解毒和降解机制对于减少农作物污染和确保食品安全至关重要。

目标

本研究旨在通过基因工程技术结合化学分析技术和代谢组学方法，创建表达水稻 ATP 结合盒（OsABC）转运蛋白基因的转基因系，阐明水稻 BNTZ 复合物在水稻中的解毒和降解途径的机制。

方法

我们建立了水稻转基因系过表达（OE）水稻 OsABC 转运蛋白及其敲除系，通过 CRISPR-Cas9 表征基因功能并测量水稻中 BNTZ 残基的积累。用 LC/Q-TOF-HRMS/MS （LIQUID CHROMATOGRAPHY/TIME OF FLIGHT HIGH RESOLUTION MASS SPECTROMETRY）对 BNTZ 的代谢物进行表征。

结果

OsABC 的过表达通过增加植株伸长率、干重和叶绿素含量，显著减少水稻组织中的细胞膜损伤和 BNTZ 积累，显著赋予水稻对 BNTZ 毒性的抗性。六种不同的代谢物和十种偶联物在化学结构中明确定义。OE 品系籽粒中降低的 BNTZ 水平和降解产物支持 OsABC 基因功能的稳健活性。使用 UPLC-Q-TOF/MS，我们进一步鉴定了各种碳水化合物、氨基酸、激素和类黄酮的积累碱性代谢物，并发现这些参与 BNTZ 降解的代谢物在 OE 品系中比在野生型（WT）水稻中增加更多。