The accumulation of combustible gases in goaf is a key and difficult point in mine disaster prevention and control, which can easily lead to coal mine accidents and seriously safety production. Methanotrophs (S2, T7) and carboxydotrophic bacteria (M13) were extracted from mine environment to cocultivation. The cultivation conditions such as inoculation, temperature, and pH were selected to explore the effects of the growth and metabolic characteristics, and reveal the reaction kinetics of mixed strains (S2/M13, T7/M13). The results showed that the optimal inoculation amount for mixed strains was 5 %, and the maximum range of mixed carbon source inoculation for S2/M13 and T7/M13 were 9.69 % CH+(7.27–9.69 % CO) and 9.69 % CH+(4.35–7.27 % CO), the optimal cultivation temperatures were 25, 30 ℃, respectively, and the optimal pH was 7. In addition, the CH metabolic rates of S2/M13 and T7/M13 were 72.4 % and 85.4 %, respectively, and the CO metabolic rate reached over 95 % under the above optimal conditions, the processes of growth and metabolism of the mix cultures remained consistent. Moreover, the growth curves of the S2/M13 and T7/M13 conformed to the Logistic model, and whose attenuation coefficients () were 0.035, −0.215 d, the maximum specific growth rates () were 0.524, 0.862 d, respectively. At the same time, the reaction rate of combustible gases showed high consistency in the logarithmic and stationary phases of the mixed strains, and exhibited a good linear relationship with the consumption rate of O, the inhibitory kinetics of mixed strains on the reaction rate of combustible gases under different cultivation conditions were determined. The research results have vital theoretical and practical significance for the prevention and control of combustible gases and the safe mining in coal mines.

中文翻译：

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微生物共培养：采空区可燃气体生物修复的反应动力学


采空区可燃气体堆积是矿山灾害防治的重点和难点，极易引发煤矿事故，严重影响安全生产。从矿山环境中提取甲烷氧化菌（S2、T7）和一氧化碳营养菌（M13）进行共培养。选择接种、温度和pH等培养条件来探索其生长和代谢特性的影响，并揭示混合菌株（S2/M13、T7/M13）的反应动力学。结果表明，混合菌株的最佳接种量为5%，S2/M13和T7/M13混合碳源接种的最大范围分别为9.69%CH+(7.27-9.69%CO)和9.69%CH+(4.35- 7.27 % CO），最适培养温度分别为25、30 ℃，最适pH为7。此外，S2/M13和T7/M13的CH代谢率分别为72.4 %和85.4 %，在上述最佳条件下CO代谢率达到95%以上，混合培养物的生长和代谢过程保持一致。此外，S2/M13和T7/M13的生长曲线符合Logistic模型，其衰减系数()分别为0.035、-0.215 d，最大比生长率()分别为0.524、0.862 d。同时，混合菌株对数期和稳定期可燃气体反应速率表现出较高的一致性，与O消耗速率呈现良好的线性关系，混合菌株对可燃气体反应速率的抑制动力学测定了不同培养条件下的气体。 研究成果对于煤矿可燃气体防治和安全开采具有重要的理论和实践意义。