Axenic dietary restriction (ADR) is highly effective in extending lifespan of Caenorhabditis elegans, but its effects on healthspan improvement are less well characterized. Using transmission electron microscopy, morphometric analyses, and functional assays, we found ADR can preserve tissue ultrastructure, including the cuticle, epidermis, and intestinal lumen, and reduce age-associated pathologies like gonad degeneration, uterine tumor clusters, pharyngeal deterioration, and intestinal atrophy. However, there was no notable improvement in behavioral and functional metrics. Our results underscore that lifespan extension through ADR does not inherently translate to broad healthspan improvements.

中文翻译：

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Axenic 饮食限制对秀丽隐杆线虫年龄相关变化的影响


Axenic 饮食限制 （ADR） 在延长秀丽隐杆线虫的寿命方面非常有效，但其对健康寿命改善的影响尚不清楚。使用透射电子显微镜、形态学分析和功能测定，我们发现 ADR 可以保留组织超微结构，包括角质层、表皮和肠腔，并减少与年龄相关的病变，如性腺变性、子宫肿瘤簇、咽部恶化和肠道萎缩。然而，行为和功能指标没有显着改善。我们的研究结果强调，通过 ADR 延长寿命本身并不能转化为广泛的健康寿命改善。