Plants sense and respond to environmental cues during 24 h fluctuations in their environment. This requires the integration of internal cues such as circadian timing with environmental cues such as light and temperature to elicit cellular responses through signal transduction. However, the integration and transduction of circadian and environmental signals by plants growing in natural environments remains poorly understood. To gain insights into 24 h dynamics of environmental signaling in nature, we performed a field study of signal transduction from the nucleus to chloroplasts in a natural population of Arabidopsis halleri. Using several modeling approaches to interpret the data, we identified that the circadian clock and temperature are key regulators of this pathway under natural conditions. We identified potential time-delay steps between pathway components, and diel fluctuations in the response of the pathway to temperature cues that are reminiscent of the process of circadian gating. We found that our modeling framework can be extended to other signaling pathways that undergo diel oscillations and respond to environmental cues. This approach of combining studies of gene expression in the field with modeling allowed us to identify the dynamic integration and transduction of environmental cues, in plant cells, under naturally fluctuating diel cycles.

中文翻译：

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拟南芥自然种群的昼夜节律和环境信号整合


植物在 24 小时环境波动期间感知环境信号并做出反应。这需要将昼夜节律等内部线索与光和温度等环境线索整合起来，以通过信号转导引发细胞反应。然而，对于自然环境中生长的植物对昼夜节律和环境信号的整合和转导仍然知之甚少。为了深入了解自然界环境信号的 24 小时动态，我们对天然种群中从细胞核到叶绿体的信号转导进行了现场研究。拟南芥。使用多种建模方法来解释数据，我们发现生物钟和温度是自然条件下该途径的关键调节因素。我们确定了通路组件之间潜在的时间延迟步骤，以及通路对温度线索的响应的昼夜波动，这让人想起昼夜节律门控过程。我们发现我们的建模框架可以扩展到经历昼夜振荡并对环境线索做出反应的其他信号通路。这种将现场基因表达研究与建模相结合的方法使我们能够识别植物细胞中自然波动的昼夜循环下环境线索的动态整合和转导。