ObjectivesCollagen membranes are extensively used for guided bone regeneration procedures, primarily for horizontal bone augmentation. More recently, it has been demonstrated that collagen membranes promote bone regeneration. Present study aimed at assessing if structural modifications of collagen membranes may enhance their osteoconductive capacity.MethodsTwenty‐four adult Wistar rats were used. Bilateral calvaria defects with a diameter of 5 mm were prepared and covered with prototypes of collagen membranes (P1 or P2). The P1 membrane (positive control) presented a lower onset temperature of protein denaturation and a higher solubility than the P2 membrane (test). The contralateral defects were left uncovered (NC). After 1 and 4 weeks, the animals were euthanized. A microcomputed tomography analysis of the harvested samples was performed within and above the bony defect. Undecalcified ground sections were subjected to light microscopy and morphometric analysis.ResultsBone formation was observed starting from the circumferential borders of the defects in all groups at 1‐week of healing. The foci of ossification were observed at the periosteal and dura mater sites, with signs of collagen membrane mineralization. However, there was no statistically significant difference between the groups. At 4 weeks, remnants of the collagen fibers were embedded in the newly formed bone. In the P2 group, significantly more bone volume, more new bone, and marrow spaces compared to the NC group were observed. Furthermore, the P2 group showed more bone volume ectocranially then the P1 group.ConclusionsBone formation subjacent to a P2 membrane was superior than subjacent to the P1 membrane and significantly better compared to the control. Modifications of the physico‐chemical properties may enhance the osteoconductive competence of collagen membranes, supporting bone formation outside the bony defects.

中文翻译：

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蛋白质变性的高溶解度和较低的起始温度增加了胶原膜的骨传导能力：一项临床前体内研究


目的胶原膜广泛用于引导骨再生手术，主要用于水平骨增量。最近，已经证明胶原膜可以促进骨骼再生。本研究旨在评估胶原膜的结构修饰是否可以增强其骨传导能力。方法使用 24 只成年 Wistar 大鼠。制备直径为 5 mm 的双侧颅骨缺损，并覆盖胶原膜原型 （P1 或 P2）。P1 膜 （阳性对照） 比 P2 膜 （测试） 表现出更低的蛋白质变性起始温度和更高的溶解度。对侧缺损未被发现 （NC）。1 周和 4 周后，对动物实施安乐死。对收获的样本在骨缺损内部和上方进行显微计算机断层扫描分析。未脱钙的地面切片进行光学显微镜检查和形态学分析。结果在愈合 1 周时，所有组都从缺损的圆周边界开始观察到骨形成。在骨膜和硬脑膜部位观察到骨化病灶，有胶原膜矿化的迹象。然而，两组之间没有统计学上的显著差异。4 周时，胶原纤维的残余部分嵌入新形成的骨骼中。与 NC 组相比，在 P2 组中观察到显著更多的骨量、更多的新骨和骨髓间隙。此外，P2 组显示颅外骨体积比 P1 组多。结论 P2 膜下骨形成优于 P1 膜下，与对照相比显著好。 物理化学性质的修饰可能会增强胶原膜的骨传导能力，支持骨缺损外的骨形成。