ObjectivesCollagen membranes are extensively used for guided bone regeneration procedures, primarily for horizontal bone augmentation. More recently, it has been demonstrated that collagen membranes promote bone regeneration. Present study aimed at assessing if structural modifications of collagen membranes may enhance their osteoconductive capacity.MethodsTwenty‐four adult Wistar rats were used. Bilateral calvaria defects with a diameter of 5 mm were prepared and covered with prototypes of collagen membranes (P1 or P2). The P1 membrane (positive control) presented a lower onset temperature of protein denaturation and a higher solubility than the P2 membrane (test). The contralateral defects were left uncovered (NC). After 1 and 4 weeks, the animals were euthanized. A microcomputed tomography analysis of the harvested samples was performed within and above the bony defect. Undecalcified ground sections were subjected to light microscopy and morphometric analysis.ResultsBone formation was observed starting from the circumferential borders of the defects in all groups at 1‐week of healing. The foci of ossification were observed at the periosteal and dura mater sites, with signs of collagen membrane mineralization. However, there was no statistically significant difference between the groups. At 4 weeks, remnants of the collagen fibers were embedded in the newly formed bone. In the P2 group, significantly more bone volume, more new bone, and marrow spaces compared to the NC group were observed. Furthermore, the P2 group showed more bone volume ectocranially then the P1 group.ConclusionsBone formation subjacent to a P2 membrane was superior than subjacent to the P1 membrane and significantly better compared to the control. Modifications of the physico‐chemical properties may enhance the osteoconductive competence of collagen membranes, supporting bone formation outside the bony defects.

中文翻译：

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较高的溶解度和较低的蛋白质变性起始温度可增加胶原膜的骨传导能力：临床前体内研究


目的胶原膜广泛用于引导骨再生手术，主要用于水平骨增量。最近，已经证明胶原蛋白膜可以促进骨再生。本研究旨在评估胶原膜的结构修饰是否可以增强其骨传导能力。方法使用 24 只成年 Wistar 大鼠。制备直径为 5 mm 的双侧颅盖缺损，并用胶原膜原型（P1 或 P2）覆盖。 P1 膜（阳性对照）比 P2 膜（测试）具有更低的蛋白质变性起始温度和更高的溶解度。对侧缺陷未被覆盖（NC）。 1周和4周后，对动物实施安乐死。对骨缺损内部和上方采集的样本进行显微计算机断层扫描分析。对未脱钙的地面切片进行光学显微镜和形态分析。结果在愈合1周时，所有组均从缺损的圆周边界开始观察到骨形成。在骨膜处观察到骨化灶硬脑膜具有胶原膜矿化迹象的位点。然而，各组之间没有统计学上的显着差异。 4 周时，残余的胶原纤维嵌入新形成的骨骼中。与 NC 组相比，在 P2 组中观察到明显更多的骨量、更多的新骨和骨髓空间。此外，P2组显示出比P1组更多的外颅骨体积。结论P2膜下方的骨形成优于P1膜下方的骨形成，并且明显优于对照。 物理化学性质的改变可以增强胶原膜的骨传导能力，支持骨缺损外的骨形成。